cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI

Jiayi Zhang, Jianliang Dai, Enpeng Zhao, Yun Lin, Li Zeng, Jinzhong Chen, Huari Zheng, Yu Wang, Xin Li, Rang Ying, Yi Xie, YuMin Mao

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Peptidylarginine deiminase (PAD) catalyzes the post-translational modification of protein through the conversion of arginine to citrulline in the presence of calcium ions. Human, similar to rodents, has four isoforms of PAD (type I, II, III and IV/V), each of which is distinct in substrate specificity and tissue specific expression. In our large-scale sequencing project, we identified a new human PAD cDNA from a human fetal brain cDNA library. The putative protein encoded by this cDNA is designated hPADVI. Expression analysis of hPADVI showed that it is mainly expressed in adult human ovary and peripheral blood leukocytes. We conclude that hPADVI may be orthologous to mouse ePAD, basing on sequence comparison, chromosome localization and exon-intron structure analysis. PAD-mediated deimination of epithelial cell keratin resulting in cytoskeletal remodeling suggests a possible role for hPADVI in cytoskeletal reorganization in the egg and in early embryo development. This study describes a new important member of the human PAD family.

Original languageEnglish (US)
Pages (from-to)1051-1058
Number of pages8
JournalActa Biochimica Polonica
Volume51
Issue number4
StatePublished - 2004
Externally publishedYes

Fingerprint

Cloning
Organism Cloning
Complementary DNA
Genes
Gene Expression
Citrulline
Chromosomes
Keratins
Gene Library
Introns
Arginine
Exons
Brain
Post Translational Protein Processing
Substrate Specificity
Protein Isoforms
Proteins
Blood
Embryonic Development
Ovum

Keywords

  • ePAD
  • hPADVI
  • Ovary
  • Peptidylarginine deiminase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Zhang, J., Dai, J., Zhao, E., Lin, Y., Zeng, L., Chen, J., ... Mao, Y. (2004). cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI. Acta Biochimica Polonica, 51(4), 1051-1058.

cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI. / Zhang, Jiayi; Dai, Jianliang; Zhao, Enpeng; Lin, Yun; Zeng, Li; Chen, Jinzhong; Zheng, Huari; Wang, Yu; Li, Xin; Ying, Rang; Xie, Yi; Mao, YuMin.

In: Acta Biochimica Polonica, Vol. 51, No. 4, 2004, p. 1051-1058.

Research output: Contribution to journalArticle

Zhang, J, Dai, J, Zhao, E, Lin, Y, Zeng, L, Chen, J, Zheng, H, Wang, Y, Li, X, Ying, R, Xie, Y & Mao, Y 2004, 'cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI', Acta Biochimica Polonica, vol. 51, no. 4, pp. 1051-1058.
Zhang, Jiayi ; Dai, Jianliang ; Zhao, Enpeng ; Lin, Yun ; Zeng, Li ; Chen, Jinzhong ; Zheng, Huari ; Wang, Yu ; Li, Xin ; Ying, Rang ; Xie, Yi ; Mao, YuMin. / cDNA cloning, gene organization and expression analysis of human peptidylarginine deiminase type VI. In: Acta Biochimica Polonica. 2004 ; Vol. 51, No. 4. pp. 1051-1058.
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