cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells

M. B. Ladner, G. A. Martin, J. A. Noble, V. P. Wittman, M. K. Warren, M. McGrogan, E. R. Stanley

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

A 4-kilobase and a 2-kilobase cDNA clone encoding a murine macrophage colony-stimulating factor have been isolated. Except for 2 amino acid residue differences, these two clones encode the same 520 amino acid residue protein, which is preceded by a 32-amino acid residue signal peptide. The two clones, whose molecular masses correspond to the two transcripts observed in murine L929 fibroblasts, contain 3' untranslated regions that are markedly different in sequence and length. Both clones can be expressed in COS cells and the recombinant protein is active in a mouse bone marrow colony assay.

Original languageEnglish (US)
Pages (from-to)6706-6710
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number18
StatePublished - 1988
Externally publishedYes

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Macrophage Colony-Stimulating Factor
Organism Cloning
Complementary DNA
Clone Cells
Amino Acids
COS Cells
3' Untranslated Regions
Protein Sorting Signals
Recombinant Proteins
Fibroblasts
Bone Marrow
Proteins

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Ladner, M. B., Martin, G. A., Noble, J. A., Wittman, V. P., Warren, M. K., McGrogan, M., & Stanley, E. R. (1988). cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. Proceedings of the National Academy of Sciences of the United States of America, 85(18), 6706-6710.

cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. / Ladner, M. B.; Martin, G. A.; Noble, J. A.; Wittman, V. P.; Warren, M. K.; McGrogan, M.; Stanley, E. R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 18, 1988, p. 6706-6710.

Research output: Contribution to journalArticle

Ladner, MB, Martin, GA, Noble, JA, Wittman, VP, Warren, MK, McGrogan, M & Stanley, ER 1988, 'cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 18, pp. 6706-6710.
Ladner, M. B. ; Martin, G. A. ; Noble, J. A. ; Wittman, V. P. ; Warren, M. K. ; McGrogan, M. ; Stanley, E. R. / cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1988 ; Vol. 85, No. 18. pp. 6706-6710.
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AU - McGrogan, M.

AU - Stanley, E. R.

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