cDNA cloning and expression analysis of a novel human F-box domain containing gene

Li Zeng, Shaohua Gu, Yao Li, Wenhu Wang, Yan Huang, Xin Ye, Jian Xu, Enpeng Zhao, Chaoneng Ji, Kang Ying, Yi Xie, Yumin Mao

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

F-box proteins are a large family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. Here we report that a novel member of F-box proteins, FBXO35 gene, was cloned and identified during the large-scale sequencing analysis from a human fetal brain cDNA library. FBXO35 gene shares amino acid similarity with several putative mouse genes not only in F-box domain but also in the rest of the sequence, which indicates that FBXO35 might also contain some other unknown conserved domain. RT-PCR analysis indicated that FBXO35 gene had a ubiquitously low expression pattern in most human adult tissues. According to bioinformatics analysis, the FBXO35 gene was found located in chromosome 3p21.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalMolecular Biology Reports
Volume31
Issue number1
DOIs
Publication statusPublished - Mar 1 2004

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Keywords

  • 3p21
  • Expression pattern
  • F-box
  • FBXO35
  • SCF complex
  • Ubiquitination

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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