cDNA cloning and expression analysis of a novel human F-box domain containing gene

Li Zeng, Shaohua Gu, Yao Li, Wenhu Wang, Yan Huang, Xin Ye, Jian Xu, Enpeng Zhao, Chaoneng Ji, Kang Ying, Yi Xie, Yumin Mao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

F-box proteins are a large family of eukaryotic proteins that are characterized by an approximately 40 amino acid motif. Some F-box proteins are critical for the controlled degradation of cellular regulatory proteins. Here we report that a novel member of F-box proteins, FBXO35 gene, was cloned and identified during the large-scale sequencing analysis from a human fetal brain cDNA library. FBXO35 gene shares amino acid similarity with several putative mouse genes not only in F-box domain but also in the rest of the sequence, which indicates that FBXO35 might also contain some other unknown conserved domain. RT-PCR analysis indicated that FBXO35 gene had a ubiquitously low expression pattern in most human adult tissues. According to bioinformatics analysis, the FBXO35 gene was found located in chromosome 3p21.

Original languageEnglish (US)
Pages (from-to)51-57
Number of pages7
JournalMolecular Biology Reports
Volume31
Issue number1
DOIs
StatePublished - Mar 2004
Externally publishedYes

Fingerprint

Cloning
F-Box Proteins
Organism Cloning
Complementary DNA
Genes
Amino Acids
Amino Acid Motifs
Bioinformatics
Chromosomes
Computational Biology
Gene Library
Brain
Proteins
Tissue
Degradation
Polymerase Chain Reaction

Keywords

  • 3p21
  • Expression pattern
  • F-box
  • FBXO35
  • SCF complex
  • Ubiquitination

ASJC Scopus subject areas

  • Genetics
  • Cell Biology
  • Biochemistry

Cite this

cDNA cloning and expression analysis of a novel human F-box domain containing gene. / Zeng, Li; Gu, Shaohua; Li, Yao; Wang, Wenhu; Huang, Yan; Ye, Xin; Xu, Jian; Zhao, Enpeng; Ji, Chaoneng; Ying, Kang; Xie, Yi; Mao, Yumin.

In: Molecular Biology Reports, Vol. 31, No. 1, 03.2004, p. 51-57.

Research output: Contribution to journalArticle

Zeng, L, Gu, S, Li, Y, Wang, W, Huang, Y, Ye, X, Xu, J, Zhao, E, Ji, C, Ying, K, Xie, Y & Mao, Y 2004, 'cDNA cloning and expression analysis of a novel human F-box domain containing gene', Molecular Biology Reports, vol. 31, no. 1, pp. 51-57. https://doi.org/10.1023/B:MOLE.0000013502.72069.19
Zeng, Li ; Gu, Shaohua ; Li, Yao ; Wang, Wenhu ; Huang, Yan ; Ye, Xin ; Xu, Jian ; Zhao, Enpeng ; Ji, Chaoneng ; Ying, Kang ; Xie, Yi ; Mao, Yumin. / cDNA cloning and expression analysis of a novel human F-box domain containing gene. In: Molecular Biology Reports. 2004 ; Vol. 31, No. 1. pp. 51-57.
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