cDNA cloning and characterization of interleukin 2-induced genes in a cloned T helper lymphocyte

Daniel E. Sabath, Patricia L. Podolin, Paul G. Comber, Michael B. Prystowsky

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Clonal expansion of antigen-specific lymphocytes is an important aspect of the immune response. Interleukin 2 (IL2) is largely responsible for the amplification of antigen-specific T cells. In this study, the changes in gene expression accompanying interleukin 2 stimulation of T cells are examined, using a cloned T helper lymphocyte line as a model system. To isolate cDNA clones of IL2-induced genes, a cDNA library was screened by differential hybridization. Twenty-one different cDNA clones were isolated by this method, comprising six glycolytic enzymes, vimentin, α-tubulin, β-actin, γ-actin, ERp99, elongation factor 2, ribosomal phosphoprotein P1, the DNA-binding protein dbpB/YB-1, as well as seven clones which do not correspond to any previously described sequences. These clones are used to study the time course of expression and the sensitivity to cycloheximide inhibition of IL2-induced mRNAs. In addition, the tissue specificity of the unidentified mRNAs is examined, and two of these are shown to be expressed at high levels in normal mouse brain, with much lower or undetectable levels in the other tissues tested. These cDNA clones will be useful in future studies to determine the molecular basis of IL2-induced gene expression.

Original languageEnglish (US)
Pages (from-to)12671-12678
Number of pages8
JournalJournal of Biological Chemistry
Volume265
Issue number21
StatePublished - Jul 25 1990
Externally publishedYes

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Lymphocytes
Cloning
Helper-Inducer T-Lymphocytes
Interleukin-2
Organism Cloning
Complementary DNA
Clone Cells
Genes
T-cells
Gene expression
Actins
Peptide Elongation Factor 2
Tissue
T-Lymphocytes
Gene Expression
Antigens
Messenger RNA
Organ Specificity
DNA-Binding Proteins
Vimentin

ASJC Scopus subject areas

  • Biochemistry

Cite this

cDNA cloning and characterization of interleukin 2-induced genes in a cloned T helper lymphocyte. / Sabath, Daniel E.; Podolin, Patricia L.; Comber, Paul G.; Prystowsky, Michael B.

In: Journal of Biological Chemistry, Vol. 265, No. 21, 25.07.1990, p. 12671-12678.

Research output: Contribution to journalArticle

Sabath, Daniel E. ; Podolin, Patricia L. ; Comber, Paul G. ; Prystowsky, Michael B. / cDNA cloning and characterization of interleukin 2-induced genes in a cloned T helper lymphocyte. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 21. pp. 12671-12678.
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