Cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network

Anne Müsch, David Cohen, Geri Kreitzer, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

It is well established that Rho-GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF-dependent manner, similarly to coat proteins involved in vesicle formation and to several actin-binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans-Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase-deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.

Original languageEnglish (US)
Pages (from-to)2171-2179
Number of pages9
JournalEMBO Journal
Volume20
Issue number9
DOIs
StatePublished - May 1 2001
Externally publishedYes

Keywords

  • Cdc42
  • Golgi
  • Protein transport

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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