Cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network

Anne Muesch, David Cohen, Geri Kreitzer, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

It is well established that Rho-GTPases regulate vesicle fusion and fission events at the plasma membrane through their modulatory role on the cortical actin cytoskeleton. In contrast, their effects on intracellular transport processes and actin pools are less clear. It was recently shown that cdc42 associates with the Golgi apparatus in an ARF-dependent manner, similarly to coat proteins involved in vesicle formation and to several actin-binding proteins. We report here that mutants of cdc42 inhibited the exit of basolateral proteins from the trans-Golgi network (TGN), while stimulating the exit of an apical marker, in two different transport assays. This regulation may result from modulation of the actin cytoskeleton, as GTPase-deficient cdc42 depleted a perinuclear actin pool that rapidly exchanges with exogenous fluorescent actin.

Original languageEnglish (US)
Pages (from-to)2171-2179
Number of pages9
JournalEMBO Journal
Volume20
Issue number9
DOIs
StatePublished - May 1 2001
Externally publishedYes

Fingerprint

trans-Golgi Network
Actins
Actin Cytoskeleton
Microfilament Proteins
rho GTP-Binding Proteins
Proteins
GTP Phosphohydrolases
Capsid Proteins
Golgi Apparatus
Cell Membrane
Cell membranes
Assays
Fusion reactions
Modulation

Keywords

  • Cdc42
  • Golgi
  • Protein transport

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network. / Muesch, Anne; Cohen, David; Kreitzer, Geri; Rodriguez-Boulan, Enrique.

In: EMBO Journal, Vol. 20, No. 9, 01.05.2001, p. 2171-2179.

Research output: Contribution to journalArticle

Muesch, Anne ; Cohen, David ; Kreitzer, Geri ; Rodriguez-Boulan, Enrique. / Cdc42 regulates the exit of apical and basolateral proteins from the trans-Golgi network. In: EMBO Journal. 2001 ; Vol. 20, No. 9. pp. 2171-2179.
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