CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture

Yakov Peter, Namita Sen, Elena Levantini, Steven Keller, Edward P. Ingenito, Aaron Ciner, Robert Sackstein, Steven D. Shapiro

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Compensatory growth is mediated by multiple cell types that interact during organ repair. To elucidate the relationship between stem/progenitor cells that proliferate or differentiate and somatic cells of the lung, we used a novel organotypic ex vivo pneumoexplant system. Applying this technique, we identified a sustained culture of repopulating adult progenitors in the form of free-floating anchorage-independent cells (AICs). AICs did not express integrin proteins α5, β3 and β7, and constituted 37% of the total culture at day 14, yielding a mixed yet conservative population that recapitulated RNA expression patterns of the healthy lung. AICs exhibited rapid proliferation manifested by a marked 60-fold increase in cell numbers by day 21. More than 50% of the AIC population was c-KIT+ or double-positive for CD45+ and CD11b+ antigenic determinants, consistent with cells of hematopoietic origin. The latter subset was found to be enriched with prosurfactant protein-C and SCGB1A1 expressing putative stem cells and with aquaporin-5 producing cells, characteristic of terminally differentiated alveolar epithelial type-1 pneumocytes. At the air/gel interface, AICs undergo remodeling to form a cellular lining, whereas TGF(β)1 treatment modifies protein expression properties to further imply a robust effect of the microenvironment on AIC phenotypic changes. These data confirm the active participation of clonogenic hematopoietic stem cells in a mammalian model of lung repair and validate mixed stem/somatic cell cultures, which license sustained cell viability, proliferation and differentiation, for use in studies of compensatory pulmonary growth.

Original languageEnglish (US)
Pages (from-to)572-583
Number of pages12
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume7
Issue number7
DOIs
StatePublished - Jul 2013

Fingerprint

Stem cells
Cell culture
Stem Cells
Cell Culture Techniques
Epithelial Cells
Lung
Proteins
Aquaporin 5
Repair
Cell proliferation
Protein C
RNA
Linings
Integrins
Epitopes
Gels
Cells
Alveolar Epithelial Cells
Air
Licensure

Keywords

  • Anchorage-independent cells
  • CD45/CD11b positive cells
  • Lung progenitors
  • Suspension culture

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture. / Peter, Yakov; Sen, Namita; Levantini, Elena; Keller, Steven; Ingenito, Edward P.; Ciner, Aaron; Sackstein, Robert; Shapiro, Steven D.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 7, No. 7, 07.2013, p. 572-583.

Research output: Contribution to journalArticle

Peter, Y, Sen, N, Levantini, E, Keller, S, Ingenito, EP, Ciner, A, Sackstein, R & Shapiro, SD 2013, 'CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture', Journal of Tissue Engineering and Regenerative Medicine, vol. 7, no. 7, pp. 572-583. https://doi.org/10.1002/term.553
Peter, Yakov ; Sen, Namita ; Levantini, Elena ; Keller, Steven ; Ingenito, Edward P. ; Ciner, Aaron ; Sackstein, Robert ; Shapiro, Steven D. / CD45/CD11b positive subsets of adult lung anchorage-independent cells harness epithelial stem cells in culture. In: Journal of Tissue Engineering and Regenerative Medicine. 2013 ; Vol. 7, No. 7. pp. 572-583.
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