CD40 Ligation Results in Protein Kinase C-Independent Activation of ERK and JNK in Resting Murine Splenic B Cells

Yi Yang Li, Mekhine Baccam, Steven B. Waters, Jeffrey E. Pessin, Gail A. Bishop, Gary A. Koretzky

Research output: Contribution to journalArticle

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Abstract

CD40 is a 45- to 50-kDa transmembrane glycoprotein that plays an important role in B cell proliferation, survival, memory, and Ig isotype switching. How CD40 engagement couples to these distal events in B cell activation remains poorly understood. In this study, we have examined signal transduction events mediated by CD40 cross-linking in resting murine splenic B cells. In comparison to signaling via the B cell Ag receptor (BCR), CD40 cross-linking was less effective at activating protein tyrosine kinases. Interestingly, however, CD40 engagement resulted in the phosphorylation of both extracellular signal-regulated protein kinase (ERK) and the Ras guanine nucleotide exchange factor, Son of sevenless. In addition, both ERK and c-Jun NH2-terminal kinase activities were increased after both CD40 and BCR ligation. Overnight treatment of cells with phorbol ester as well as pharmacologic inhibitors of protein kinase C abrogated these signaling events after BCR treatment; however, no effect was seen on CD40-mediated activation of ERK or c-Jun NH2-terminal kinase, suggesting that the BCR and CD40 differentially utilize protein kinase C to couple with these signaling pathways.

Original languageEnglish (US)
Pages (from-to)1440-1447
Number of pages8
JournalJournal of Immunology
Volume157
Issue number4
StatePublished - Aug 15 1996
Externally publishedYes

Fingerprint

Protein Kinase C
Ligation
B-Lymphocytes
JNK Mitogen-Activated Protein Kinases
ras Guanine Nucleotide Exchange Factors
Immunoglobulin Class Switching
Extracellular Signal-Regulated MAP Kinases
Phorbol Esters
Nuclear Family
Protein-Tyrosine Kinases
Protein Kinases
Signal Transduction
Cell Survival
Glycoproteins
Phosphorylation
Cell Proliferation

ASJC Scopus subject areas

  • Immunology

Cite this

Li, Y. Y., Baccam, M., Waters, S. B., Pessin, J. E., Bishop, G. A., & Koretzky, G. A. (1996). CD40 Ligation Results in Protein Kinase C-Independent Activation of ERK and JNK in Resting Murine Splenic B Cells. Journal of Immunology, 157(4), 1440-1447.

CD40 Ligation Results in Protein Kinase C-Independent Activation of ERK and JNK in Resting Murine Splenic B Cells. / Li, Yi Yang; Baccam, Mekhine; Waters, Steven B.; Pessin, Jeffrey E.; Bishop, Gail A.; Koretzky, Gary A.

In: Journal of Immunology, Vol. 157, No. 4, 15.08.1996, p. 1440-1447.

Research output: Contribution to journalArticle

Li, YY, Baccam, M, Waters, SB, Pessin, JE, Bishop, GA & Koretzky, GA 1996, 'CD40 Ligation Results in Protein Kinase C-Independent Activation of ERK and JNK in Resting Murine Splenic B Cells', Journal of Immunology, vol. 157, no. 4, pp. 1440-1447.
Li, Yi Yang ; Baccam, Mekhine ; Waters, Steven B. ; Pessin, Jeffrey E. ; Bishop, Gail A. ; Koretzky, Gary A. / CD40 Ligation Results in Protein Kinase C-Independent Activation of ERK and JNK in Resting Murine Splenic B Cells. In: Journal of Immunology. 1996 ; Vol. 157, No. 4. pp. 1440-1447.
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