CD4 confers susceptibility to human immunodeficiency virus type 1 infection in a rat fibroblast cell line

Yaffa Mizrachi, Arye Rubinstein, Mala Golodner, Koji Sakai, David J. Volsky

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A new model system is delineated that will enable study of CD4 cofactors and gp120 binding proteins other than CD4. We have previously described a nontransformed rat fibroblast cell line that can efficiently produce HIV-1 upon transfection with an HIV-1 infectious clone, in contrast to other nonhuman mammalian cell lines tested. In the present study we analyzed the susceptibility to HIV-1 infection of Rat2 cells expressing the human CD4 protein. We have used the mammalian expression vector pKS286, in which HIV-1 LTR drives the expression of the CD4 gene. We show that the Rat2 cell line, expressing the human CD4 (Rat2/CD4), is susceptible to fusion with and infection by HIV-1. The virus produced by the Rat2/CD4 cells was infectious. CD4 expression in the Rat2/CD4 was down-regulated over time, similarly to HIV-1 expression in HIV-1-transfected Rat2 cells. Transfection of the Rat2/CD4 cells with a tat expression vector reestablished the CD4 expression on the surface of those cells, as expected. We conclude that the expression of a gp120 binding protein on the Rat2 cells' surface suffices to render the Rat2 cells susceptible to HIV-1 infection.

Original languageEnglish (US)
Pages (from-to)1315-1318
Number of pages4
JournalAIDS Research and Human Retroviruses
Volume12
Issue number14
DOIs
StatePublished - Sep 20 1996

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ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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