CD39/NTPDase-1 activity and expression in normal leukocytes

E. Dianne Pulte, M. Johan Broekman, Kim E. Olson, Joan H F Drosopoulos, Jorge Kizer, Naziba Islam, Aaron J. Marcus

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Introduction: CD39/NTPDase-1 is a cell surface enzyme expressed on leukocytes and endothelial cells that metabolizes ATP to ADP and AMP. CD39 is expressed on numerous different types of normal leukocytes, but details of its expression have not been determined previously. Methods: We examined CD39 expression and activity in leukocytes isolated from healthy volunteers. Expression of CD39 on leukocytes was measured by FACS and activity of CD39 in lymphocytes and neutrophils was determined by an enzymatic radio-TLC assay. Results: We established that CD39 is expressed on neutrophils, lymphocytes, and monocytes. The enzyme is found on > 90% of monocytes, neutrophils, and B-lymphocytes, and 6% of T-lymphocytes and natural killer cells. Per cell density of expression varied, with the highest expression on monocytes and B-lymphocytes. ATPase and ADPase activities were highest on B-lymphocytes, lower on neutrophils, lowest on T-lymphocytes. The ratio of ADPase:ATPase activity was 1.8 for neutrophils and B-lymphocytes and 1.4 for T-lymphocytes. Hypertensive volunteers had lower levels of CD39 on their T-lymphocytes and NK cells. No correlation between age, gender, ethnic background, or cholesterol level and CD39 expression was observed. Conclusions: We conclude that CD39 activity and expression are present to varying degrees on all leukocytes types examined. Differences between leukocyte types should be considered when examining CD39 in disease states.

Original languageEnglish (US)
Pages (from-to)309-317
Number of pages9
JournalThrombosis Research
Volume121
Issue number3
DOIs
StatePublished - 2007
Externally publishedYes

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Leukocytes
Neutrophils
B-Lymphocytes
Apyrase
T-Lymphocytes
Monocytes
Natural Killer Cells
Adenosine Triphosphatases
Lymphocytes
Enzymes
Adenosine Monophosphate
Radio
Adenosine Diphosphate
CD39 antigen
Volunteers
Healthy Volunteers
Endothelial Cells
Cell Count
Adenosine Triphosphate
Cholesterol

Keywords

  • CD39
  • Lymphocytes
  • Thrombosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Pulte, E. D., Broekman, M. J., Olson, K. E., Drosopoulos, J. H. F., Kizer, J., Islam, N., & Marcus, A. J. (2007). CD39/NTPDase-1 activity and expression in normal leukocytes. Thrombosis Research, 121(3), 309-317. https://doi.org/10.1016/j.thromres.2007.04.008

CD39/NTPDase-1 activity and expression in normal leukocytes. / Pulte, E. Dianne; Broekman, M. Johan; Olson, Kim E.; Drosopoulos, Joan H F; Kizer, Jorge; Islam, Naziba; Marcus, Aaron J.

In: Thrombosis Research, Vol. 121, No. 3, 2007, p. 309-317.

Research output: Contribution to journalArticle

Pulte, ED, Broekman, MJ, Olson, KE, Drosopoulos, JHF, Kizer, J, Islam, N & Marcus, AJ 2007, 'CD39/NTPDase-1 activity and expression in normal leukocytes', Thrombosis Research, vol. 121, no. 3, pp. 309-317. https://doi.org/10.1016/j.thromres.2007.04.008
Pulte ED, Broekman MJ, Olson KE, Drosopoulos JHF, Kizer J, Islam N et al. CD39/NTPDase-1 activity and expression in normal leukocytes. Thrombosis Research. 2007;121(3):309-317. https://doi.org/10.1016/j.thromres.2007.04.008
Pulte, E. Dianne ; Broekman, M. Johan ; Olson, Kim E. ; Drosopoulos, Joan H F ; Kizer, Jorge ; Islam, Naziba ; Marcus, Aaron J. / CD39/NTPDase-1 activity and expression in normal leukocytes. In: Thrombosis Research. 2007 ; Vol. 121, No. 3. pp. 309-317.
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AU - Marcus, Aaron J.

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