CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice

Victor A. Drover, David V. Nguyen, Claire C. Bastie, Yolanda F. Darlington, Nada A. Abumrad, Jeffrey E. Pessin, Erwin London, Daisy Sahoo, Michael C. Phillips

Research output: Contribution to journalArticle

93 Scopus citations

Abstract

The intestine has an extraordinary capacity for fatty acid (FA) absorption. Numerous candidates for a protein-mediated mechanism of dietary FA absorption have been proposed, but firm evidence for this process has remained elusive. Here we show that the scavenger receptor CD36 is required both for the uptake of very long chain FAs (VLCFAs) in cultured cells and the absorption of dietary VLCFAs in mice. We found that the fraction of CD36-dependent saturated fatty acid association/absorption in these model systems is proportional to the FA chain length and specific for fatty acids and fatty alcohols containing very long saturated acyl chains. Moreover, intestinal VLCFA absorption is completely abolished in CD36-null mice fed a high fat diet, illustrating that the predominant mechanism for VLCFA absorption is CD36-dependent. Together, these findings represent the first direct evidence for protein-facilitated FA absorption in the intestine and identify a novel therapeutic target for the treatment of diseases characterized by elevated VLCFA levels.

Original languageEnglish (US)
Pages (from-to)13108-13115
Number of pages8
JournalJournal of Biological Chemistry
Volume283
Issue number19
DOIs
StatePublished - May 9 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Drover, V. A., Nguyen, D. V., Bastie, C. C., Darlington, Y. F., Abumrad, N. A., Pessin, J. E., London, E., Sahoo, D., & Phillips, M. C. (2008). CD36 mediates both cellular uptake of very long chain fatty acids and their intestinal absorption in mice. Journal of Biological Chemistry, 283(19), 13108-13115. https://doi.org/10.1074/jbc.M708086200