CD3ζ/η/θ locus is colinear with and transcribed antisense to the gene encoding the transcription factor oct-1

Adam Lerner, Luciano D'Adamio, Andrew C. Diener, Linda K. Clayton, Ellis L. Reinherz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

CD3ζ and η are signal-transducing components of the TCR and are derived from alternative splicing of transcripts from a single genetic locus that also encodes CD3θ. We have isolated two murine cDNA clones that appear to result from antisense transcription through CD3θ-specific exon 10 and CD3η-specific exon 9. The sequence of these clones shows no open reading frame. Northern analysis with single stranded probes confirms the existence of a ubiquitously expressed >12-kb polyadenylated mRNA antisense to CD3η. A "genomic walk," which extended 32 kb distal to murine CD3η exon 9, provided genomic DNA containing a more 5′ portion of the antisense transcript. This probe identified two murine thymic cDNA with 91% sequence homology to the human transcription factor Oct-1. Five exons of murine Oct-1 map in an antisense orientation to the CD3ζ/η/θ) locus on the cloned genomic sequences. The murine Oct-1 cDNA and exon 9 of CD3η hybridize to the same >12-kb mRNA. Similarly, human Oct-1 and previously characterized human genomic sequences homologous to murine CD3η exon 9 each hybridize to the same >15-kb human mRNA. Thus, the CD3ζ/η/θ and Oct-1 gene loci are partially overlapping and transcribed in opposite directions. The potential functional implications of these findings are discussed. Journal of Immunology, 1993, 151: 3152.

Original languageEnglish (US)
Pages (from-to)3152-3162
Number of pages11
JournalJournal of Immunology
Volume151
Issue number6
StatePublished - 1993
Externally publishedYes

Fingerprint

Octamer Transcription Factor-1
Exons
Genes
Complementary DNA
Sequence Homology
Messenger RNA
Clone Cells
Genetic Loci
Alternative Splicing
Allergy and Immunology
Open Reading Frames
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Lerner, A., D'Adamio, L., Diener, A. C., Clayton, L. K., & Reinherz, E. L. (1993). CD3ζ/η/θ locus is colinear with and transcribed antisense to the gene encoding the transcription factor oct-1. Journal of Immunology, 151(6), 3152-3162.

CD3ζ/η/θ locus is colinear with and transcribed antisense to the gene encoding the transcription factor oct-1. / Lerner, Adam; D'Adamio, Luciano; Diener, Andrew C.; Clayton, Linda K.; Reinherz, Ellis L.

In: Journal of Immunology, Vol. 151, No. 6, 1993, p. 3152-3162.

Research output: Contribution to journalArticle

Lerner, A, D'Adamio, L, Diener, AC, Clayton, LK & Reinherz, EL 1993, 'CD3ζ/η/θ locus is colinear with and transcribed antisense to the gene encoding the transcription factor oct-1', Journal of Immunology, vol. 151, no. 6, pp. 3152-3162.
Lerner, Adam ; D'Adamio, Luciano ; Diener, Andrew C. ; Clayton, Linda K. ; Reinherz, Ellis L. / CD3ζ/η/θ locus is colinear with and transcribed antisense to the gene encoding the transcription factor oct-1. In: Journal of Immunology. 1993 ; Vol. 151, No. 6. pp. 3152-3162.
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N2 - CD3ζ and η are signal-transducing components of the TCR and are derived from alternative splicing of transcripts from a single genetic locus that also encodes CD3θ. We have isolated two murine cDNA clones that appear to result from antisense transcription through CD3θ-specific exon 10 and CD3η-specific exon 9. The sequence of these clones shows no open reading frame. Northern analysis with single stranded probes confirms the existence of a ubiquitously expressed >12-kb polyadenylated mRNA antisense to CD3η. A "genomic walk," which extended 32 kb distal to murine CD3η exon 9, provided genomic DNA containing a more 5′ portion of the antisense transcript. This probe identified two murine thymic cDNA with 91% sequence homology to the human transcription factor Oct-1. Five exons of murine Oct-1 map in an antisense orientation to the CD3ζ/η/θ) locus on the cloned genomic sequences. The murine Oct-1 cDNA and exon 9 of CD3η hybridize to the same >12-kb mRNA. Similarly, human Oct-1 and previously characterized human genomic sequences homologous to murine CD3η exon 9 each hybridize to the same >15-kb human mRNA. Thus, the CD3ζ/η/θ and Oct-1 gene loci are partially overlapping and transcribed in opposite directions. The potential functional implications of these findings are discussed. Journal of Immunology, 1993, 151: 3152.

AB - CD3ζ and η are signal-transducing components of the TCR and are derived from alternative splicing of transcripts from a single genetic locus that also encodes CD3θ. We have isolated two murine cDNA clones that appear to result from antisense transcription through CD3θ-specific exon 10 and CD3η-specific exon 9. The sequence of these clones shows no open reading frame. Northern analysis with single stranded probes confirms the existence of a ubiquitously expressed >12-kb polyadenylated mRNA antisense to CD3η. A "genomic walk," which extended 32 kb distal to murine CD3η exon 9, provided genomic DNA containing a more 5′ portion of the antisense transcript. This probe identified two murine thymic cDNA with 91% sequence homology to the human transcription factor Oct-1. Five exons of murine Oct-1 map in an antisense orientation to the CD3ζ/η/θ) locus on the cloned genomic sequences. The murine Oct-1 cDNA and exon 9 of CD3η hybridize to the same >12-kb mRNA. Similarly, human Oct-1 and previously characterized human genomic sequences homologous to murine CD3η exon 9 each hybridize to the same >15-kb human mRNA. Thus, the CD3ζ/η/θ and Oct-1 gene loci are partially overlapping and transcribed in opposite directions. The potential functional implications of these findings are discussed. Journal of Immunology, 1993, 151: 3152.

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