CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes

M. Exley, J. Garcia, S. B. Wilson, F. Spada, D. Gerdes, S. M A Tahir, K. T. Patton, R. S. Blumberg, Steven A. Porcelli, A. Chott, Steven P. Balk

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Abstract

Human T cells expressing CD161 and an invariant T-cell receptor (TCR) α-chain (Vα24(invt) T cells) specifically recognize CD1d and appear to have immunoregulatory functions. However, the physiological target cells for this T-cell population, and whether alterations in CD1d expression contribute to the regulation of Vα24(invt) T-cell responses, remain to be determined. A series of antibodies were generated to assess CD1d expression, structure and regulation on human lymphoid and myeloid cells. CD1d was expressed at high levels by human cortical thymocytes and immunoprecipitation analyses showed it to be a 48,000-MW glycosylated protein. However, after solubilization, the majority of the thymocyte CD1d protein, but not CD1d expressed by transfected cells, lost reactivity with monoclonal antibodies (mAbs) against native CD1d, indicating that it was alternatively processed. Moreover, thymocytes were not recognized by CD1d-reactive Vα24(invt) T-cell clones. Medullary thymocytes and resting peripheral blood T cells were CD1d, but low-level CD1d expression was induced on activated T cells. CD1d was expressed by B cells in peripheral blood and lymph node mantle zones, but germinal centres were CD1d. Resting monocytes were CD1d+ but, in contrast to CD1a, b and c, their surface expression of CD1d was not up-regulated by granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) activation. These results demonstrate constitutive CD1d expression by human professional antigen-presenting cells and that post-translational processing of CD1d may contribute to regulation of the activity of CD1d-specific T cells.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalImmunology
Volume100
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

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Thymocytes
Monocytes
Blood Cells
B-Lymphocytes
T-Lymphocytes
Germinal Center
Antigen-Presenting Cells
Myeloid Cells
Granulocyte-Macrophage Colony-Stimulating Factor
T-Cell Antigen Receptor
Immunoprecipitation
Interleukin-4
Proteins
Clone Cells
Lymph Nodes
Monoclonal Antibodies
Lymphocytes
Antibodies
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Exley, M., Garcia, J., Wilson, S. B., Spada, F., Gerdes, D., Tahir, S. M. A., ... Balk, S. P. (2000). CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. Immunology, 100(1), 37-47. https://doi.org/10.1046/j.1365-2567.2000.00001.x

CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. / Exley, M.; Garcia, J.; Wilson, S. B.; Spada, F.; Gerdes, D.; Tahir, S. M A; Patton, K. T.; Blumberg, R. S.; Porcelli, Steven A.; Chott, A.; Balk, Steven P.

In: Immunology, Vol. 100, No. 1, 2000, p. 37-47.

Research output: Contribution to journalArticle

Exley, M, Garcia, J, Wilson, SB, Spada, F, Gerdes, D, Tahir, SMA, Patton, KT, Blumberg, RS, Porcelli, SA, Chott, A & Balk, SP 2000, 'CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes', Immunology, vol. 100, no. 1, pp. 37-47. https://doi.org/10.1046/j.1365-2567.2000.00001.x
Exley, M. ; Garcia, J. ; Wilson, S. B. ; Spada, F. ; Gerdes, D. ; Tahir, S. M A ; Patton, K. T. ; Blumberg, R. S. ; Porcelli, Steven A. ; Chott, A. ; Balk, Steven P. / CD1d structure and regulation on human thymocytes, peripheral blood T cells, B cells and monocytes. In: Immunology. 2000 ; Vol. 100, No. 1. pp. 37-47.
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AU - Wilson, S. B.

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AU - Gerdes, D.

AU - Tahir, S. M A

AU - Patton, K. T.

AU - Blumberg, R. S.

AU - Porcelli, Steven A.

AU - Chott, A.

AU - Balk, Steven P.

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