CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease

Peter A. Sieling, Steven A. Porcelli, Franca Spada, Marika Falcone, Barry R. Bloom, Betty Diamond, Robert L. Modlin

Research output: Contribution to journalArticle

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Abstract

The role of CD1-restricted T cells in autoimmunity was investigated by studying patients with systemic lupus erythematosus (SLE). CD4-.CD8- double negative (DN) T-cell lines were derived from the peripheral blood of patients with SLE and from healthy donors in the presence of CD1+ antigen presenting cells. Double negative T-cell lines were restricted by CD1c and produced IL-4 but not IFN-γ. In contrast, CD1c-reactive T-cells from healthy donors produced no IL-4 and produced considerable amounts of IFN-γ. B-lymphoblastoid cell lines transfected with CD1c and CD1c+ peripheral blood B-cells induced IL-4 production from SLE-derived double negative T-cell lines, suggesting that cognate recognition of a self antigen could take place in the context of CD1c. Finally, CD1c-reactive T-cells responded to CD1c lacking an endosomal. targeting signal, indicating that antigen presentation is independent of endosomal processing. These data suggest that CD1c-reactive, Th2 T cells in SLE could interact with B cells and alter antibody responses in autoimmune disease.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

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T-cells
autoimmune diseases
Autoimmune Diseases
cytokines
T-lymphocytes
lupus erythematosus
Cytokines
T-Lymphocytes
Systemic Lupus Erythematosus
interleukin-4
cell lines
Interleukin-4
B-lymphocytes
Cell Line
Cells
CD1 Antigens
Blood
B-Lymphocytes
Tissue Donors
antigen presentation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Sieling, P. A., Porcelli, S. A., Spada, F., Falcone, M., Bloom, B. R., Diamond, B., & Modlin, R. L. (1998). CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease. FASEB Journal, 12(5).

CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease. / Sieling, Peter A.; Porcelli, Steven A.; Spada, Franca; Falcone, Marika; Bloom, Barry R.; Diamond, Betty; Modlin, Robert L.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Sieling, PA, Porcelli, SA, Spada, F, Falcone, M, Bloom, BR, Diamond, B & Modlin, RL 1998, 'CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease', FASEB Journal, vol. 12, no. 5.
Sieling PA, Porcelli SA, Spada F, Falcone M, Bloom BR, Diamond B et al. CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease. FASEB Journal. 1998 Mar 20;12(5).
Sieling, Peter A. ; Porcelli, Steven A. ; Spada, Franca ; Falcone, Marika ; Bloom, Barry R. ; Diamond, Betty ; Modlin, Robert L. / CD1c-reactive, Th2 cytokine producing T-cells in human autoimmune disease. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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