CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells

Stephanie C. Degner, Jiyoti Verma-Gaur, Timothy P. Wong, Claudia Bossen, G. Michael Iverson, Ali Torkamani, Christian Vettermann, Yin C. Lin, Zhongliang Ju, Danae Schulz, Caroline S. Murre, Barbara K. Birshtein, Nicholas J. Schork, Mark S. Schlissel, Roy Riblet, Cornelis Murre, Ann J. Feeney

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Compaction and looping of the ∼2.5-Mb Igh locus during V(D)J rearrangement is essential to allow all VH genes to be brought in proximity with DH-JH segments to create a diverse antibody repertoire, but the proteins directly responsible for this are unknown. Because CCCTC-binding factor (CTCF) has been demonstrated to be involved in long-range chromosomal interactions, we hypothesized that CTCF may promote the contraction of the Igh locus. ChIP sequencing was performed on pro-B cells, revealing colocalization of CTCF and Rad21 binding at ∼60 sites throughout the V H region and 2 other sites within the Igh locus. These numerous CTCF/cohesin sites potentially form the bases of the multiloop rosette structures at the Igh locus that compact during Ig heavy chain rearrangement. To test whether CTCF was involved in locus compaction, we used 3D-FISH to measure compaction in pro-B cells transduced with CTCF shRNA retroviruses. Reduction of CTCF binding resulted in a decrease in Igh locus compaction. Long-range interactions within the Igh locus were measured with the chromosomal conformation capture assay, revealing direct interactions between CTCF sites 5′ of DFL16 and the 3′ regulatory region, and also the intronic enhancer (Eμ), creating a DH-JH-Eμ-CH domain. Knockdown of CTCF also resulted in the increase of antisense transcription throughout the DH region and parts of the VH locus, suggesting a widespread regulatory role for CTCF. Together, our findings demonstrate that CTCF plays an important role in the 3D structure of the Igh locus and in the regulation of antisense germline transcription and that it contributes to the compaction of the Igh locus.

Original languageEnglish (US)
Pages (from-to)9566-9571
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number23
DOIs
StatePublished - Jun 7 2011

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B-Lymphoid Precursor Cells
Immunoglobulin Heavy Chains
Nucleic Acid Regulatory Sequences
Retroviridae
Small Interfering RNA
Antibodies
Genes
Proteins
CCCTC-binding factor
cohesins

Keywords

  • Boundary element
  • ChIP-seq
  • Insulator
  • Lymphocytes
  • V(D)J recombination

ASJC Scopus subject areas

  • General

Cite this

CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells. / Degner, Stephanie C.; Verma-Gaur, Jiyoti; Wong, Timothy P.; Bossen, Claudia; Iverson, G. Michael; Torkamani, Ali; Vettermann, Christian; Lin, Yin C.; Ju, Zhongliang; Schulz, Danae; Murre, Caroline S.; Birshtein, Barbara K.; Schork, Nicholas J.; Schlissel, Mark S.; Riblet, Roy; Murre, Cornelis; Feeney, Ann J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 23, 07.06.2011, p. 9566-9571.

Research output: Contribution to journalArticle

Degner, SC, Verma-Gaur, J, Wong, TP, Bossen, C, Iverson, GM, Torkamani, A, Vettermann, C, Lin, YC, Ju, Z, Schulz, D, Murre, CS, Birshtein, BK, Schork, NJ, Schlissel, MS, Riblet, R, Murre, C & Feeney, AJ 2011, 'CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 23, pp. 9566-9571. https://doi.org/10.1073/pnas.1019391108
Degner, Stephanie C. ; Verma-Gaur, Jiyoti ; Wong, Timothy P. ; Bossen, Claudia ; Iverson, G. Michael ; Torkamani, Ali ; Vettermann, Christian ; Lin, Yin C. ; Ju, Zhongliang ; Schulz, Danae ; Murre, Caroline S. ; Birshtein, Barbara K. ; Schork, Nicholas J. ; Schlissel, Mark S. ; Riblet, Roy ; Murre, Cornelis ; Feeney, Ann J. / CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense transcription in pro-B cells. In: Proceedings of the National Academy of Sciences of the United States of America. 2011 ; Vol. 108, No. 23. pp. 9566-9571.
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AU - Bossen, Claudia

AU - Iverson, G. Michael

AU - Torkamani, Ali

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AU - Lin, Yin C.

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AU - Schulz, Danae

AU - Murre, Caroline S.

AU - Birshtein, Barbara K.

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