Caveolin-1-deficient mice show defects in innate immunity and inflammatory immune response during Salmonella enterica serovar typhimurium infection

Freddy A. Medina, Cecilia J. De Almeida, Elliott Dew, Jiangwei Li, Gloria Bonuccelli, Terence M. Williams, Alex W. Cohen, Richard G. Pestell, Philippe G. Frank, Herbert B. Tanowitz, Michael P. Lisanti

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A number of studies have shown an association of pathogens with caveolae. To this date, however, there are no studies showing a role for caveolin-1 in modulating immune responses against pathogens. Interestingly, expression of caveolin-1 has been shown to occur in a regulated manner in immune cells in response to lipopolysaccharide (LPS). Here, we sought to determine the role of caveolin-1 (Cav-1) expression in Salmonella pathogenesis. Cav-1-/- mice displayed a significant decrease in survival when challenged with Salmonella enterica serovar Typhimurium. Spleen and tissue burdens were significantly higher in Cav-1-/- mice. However, infection of Cav-1-/- macrophages with serovar Typhimurium did not esult in differences in bacterial invasion. In addition, Cav-1-/- mice displayed increased production of inflammatory cytokines, chemokines, and nitric oxide. Regardless of this, Cav-1-/- mice were unable to control the systemic infection of Salmonella. The increased chemokine production in Cav-1-/- mice resulted in greater infiltration of neutrophils into granulomas but did not alter the number of granulomas present. This was accompanied by increased necrosis in the liver. However, Cav-1-/- macrophages displayed increased inflammatory responses and increased nitric oxide production in vitro in response to Salmonella LPS. These results show that caveolin-1 plays a key role in regulating anti-inflammatory responses in macrophages. Taken together, these data suggest that the increased production of toxic mediators from macrophages lacking caveolin-1 is likely to be responsible for the marked susceptibility of caveolin-1-deficient mice to S. enterica serovar Typhimurium.

Original languageEnglish (US)
Pages (from-to)6665-6674
Number of pages10
JournalInfection and Immunity
Volume74
Issue number12
DOIs
StatePublished - Dec 2006

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Caveolin 1
Salmonella enterica
Innate Immunity
Infection
Macrophages
Granuloma
Chemokines
Salmonella
Serogroup
Lipopolysaccharides
Nitric Oxide
Caveolae
Neutrophil Infiltration
Salmonella Infections
Poisons
Infection Control

ASJC Scopus subject areas

  • Immunology

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Caveolin-1-deficient mice show defects in innate immunity and inflammatory immune response during Salmonella enterica serovar typhimurium infection. / Medina, Freddy A.; De Almeida, Cecilia J.; Dew, Elliott; Li, Jiangwei; Bonuccelli, Gloria; Williams, Terence M.; Cohen, Alex W.; Pestell, Richard G.; Frank, Philippe G.; Tanowitz, Herbert B.; Lisanti, Michael P.

In: Infection and Immunity, Vol. 74, No. 12, 12.2006, p. 6665-6674.

Research output: Contribution to journalArticle

Medina, FA, De Almeida, CJ, Dew, E, Li, J, Bonuccelli, G, Williams, TM, Cohen, AW, Pestell, RG, Frank, PG, Tanowitz, HB & Lisanti, MP 2006, 'Caveolin-1-deficient mice show defects in innate immunity and inflammatory immune response during Salmonella enterica serovar typhimurium infection', Infection and Immunity, vol. 74, no. 12, pp. 6665-6674. https://doi.org/10.1128/IAI.00949-06
Medina, Freddy A. ; De Almeida, Cecilia J. ; Dew, Elliott ; Li, Jiangwei ; Bonuccelli, Gloria ; Williams, Terence M. ; Cohen, Alex W. ; Pestell, Richard G. ; Frank, Philippe G. ; Tanowitz, Herbert B. ; Lisanti, Michael P. / Caveolin-1-deficient mice show defects in innate immunity and inflammatory immune response during Salmonella enterica serovar typhimurium infection. In: Infection and Immunity. 2006 ; Vol. 74, No. 12. pp. 6665-6674.
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abstract = "A number of studies have shown an association of pathogens with caveolae. To this date, however, there are no studies showing a role for caveolin-1 in modulating immune responses against pathogens. Interestingly, expression of caveolin-1 has been shown to occur in a regulated manner in immune cells in response to lipopolysaccharide (LPS). Here, we sought to determine the role of caveolin-1 (Cav-1) expression in Salmonella pathogenesis. Cav-1-/- mice displayed a significant decrease in survival when challenged with Salmonella enterica serovar Typhimurium. Spleen and tissue burdens were significantly higher in Cav-1-/- mice. However, infection of Cav-1-/- macrophages with serovar Typhimurium did not esult in differences in bacterial invasion. In addition, Cav-1-/- mice displayed increased production of inflammatory cytokines, chemokines, and nitric oxide. Regardless of this, Cav-1-/- mice were unable to control the systemic infection of Salmonella. The increased chemokine production in Cav-1-/- mice resulted in greater infiltration of neutrophils into granulomas but did not alter the number of granulomas present. This was accompanied by increased necrosis in the liver. However, Cav-1-/- macrophages displayed increased inflammatory responses and increased nitric oxide production in vitro in response to Salmonella LPS. These results show that caveolin-1 plays a key role in regulating anti-inflammatory responses in macrophages. Taken together, these data suggest that the increased production of toxic mediators from macrophages lacking caveolin-1 is likely to be responsible for the marked susceptibility of caveolin-1-deficient mice to S. enterica serovar Typhimurium.",
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AU - De Almeida, Cecilia J.

AU - Dew, Elliott

AU - Li, Jiangwei

AU - Bonuccelli, Gloria

AU - Williams, Terence M.

AU - Cohen, Alex W.

AU - Pestell, Richard G.

AU - Frank, Philippe G.

AU - Tanowitz, Herbert B.

AU - Lisanti, Michael P.

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AB - A number of studies have shown an association of pathogens with caveolae. To this date, however, there are no studies showing a role for caveolin-1 in modulating immune responses against pathogens. Interestingly, expression of caveolin-1 has been shown to occur in a regulated manner in immune cells in response to lipopolysaccharide (LPS). Here, we sought to determine the role of caveolin-1 (Cav-1) expression in Salmonella pathogenesis. Cav-1-/- mice displayed a significant decrease in survival when challenged with Salmonella enterica serovar Typhimurium. Spleen and tissue burdens were significantly higher in Cav-1-/- mice. However, infection of Cav-1-/- macrophages with serovar Typhimurium did not esult in differences in bacterial invasion. In addition, Cav-1-/- mice displayed increased production of inflammatory cytokines, chemokines, and nitric oxide. Regardless of this, Cav-1-/- mice were unable to control the systemic infection of Salmonella. The increased chemokine production in Cav-1-/- mice resulted in greater infiltration of neutrophils into granulomas but did not alter the number of granulomas present. This was accompanied by increased necrosis in the liver. However, Cav-1-/- macrophages displayed increased inflammatory responses and increased nitric oxide production in vitro in response to Salmonella LPS. These results show that caveolin-1 plays a key role in regulating anti-inflammatory responses in macrophages. Taken together, these data suggest that the increased production of toxic mediators from macrophages lacking caveolin-1 is likely to be responsible for the marked susceptibility of caveolin-1-deficient mice to S. enterica serovar Typhimurium.

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