Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo

Tomonori Nakazato, Keisuke Ito, Yoshitaka Miyakawa, Kentaro Kinjo, Taketo Yamada, Nobumichi Hozumi, Yasuo Ikeda, Masahiro Kizaki

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Background and Objectives. The aim of this study was to investigate the possibility of green tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG) as a novel therapeutic agent for patients with myeloid leukemia. Design and Methods. We investigated the effects of EGCG on the induction of apoptosis in leukemic cells in vitro and in vivo. We further examined the molecular mechanisms of EGCG-induced apoptosis in myeloid leukemic cells. Results. EGCG rapidly induced apoptotic cell death in retinoic acid (RA)-resistant acute promyelocytic leukemia (APL), UF-1 cells within 3 h. EGCG-induced apoptosis in UF-1 cells was associated with the loss of mitochondrial transmembrane potentials (ΔΨm) and activation of caspase-3 and -9. Elevation of intracellular reactive oxygen species (ROS) production was also demonstrated during EGCG-induced apoptosis of UF-1 as well as fresh myeloid leukemic cells. In NOD/SCID mice transplanted with UF-1 cells, EGCG effectively inhibited tumor growth in vivo, and the number of mitoses among the cells significantly decreased in comparison to the number in control mouse cells. Interpretation and Conclusions. In summary, EGCG has potential as a novel therapeutic agent for myeloid leukemia via induction of apoptosis mediated by modification of the redox system.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalHaematologica
Volume90
Issue number3
StatePublished - Mar 2005
Externally publishedYes

Fingerprint

Catechin
Tea
Myeloid Cells
Reactive Oxygen Species
Apoptosis
Growth
Neoplasms
Myeloid Leukemia
Inbred NOD Mouse
Acute Promyelocytic Leukemia
SCID Mice
In Vitro Techniques
epigallocatechin gallate
Caspase 9
Polyphenols
Proxy
Tretinoin
Mitosis
Caspase 3
Membrane Potentials

Keywords

  • Apoptosis
  • Catechin
  • Green tea
  • Leukemic cells
  • Reactive oxygen species

ASJC Scopus subject areas

  • Hematology

Cite this

Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo. / Nakazato, Tomonori; Ito, Keisuke; Miyakawa, Yoshitaka; Kinjo, Kentaro; Yamada, Taketo; Hozumi, Nobumichi; Ikeda, Yasuo; Kizaki, Masahiro.

In: Haematologica, Vol. 90, No. 3, 03.2005, p. 317-325.

Research output: Contribution to journalArticle

Nakazato, Tomonori ; Ito, Keisuke ; Miyakawa, Yoshitaka ; Kinjo, Kentaro ; Yamada, Taketo ; Hozumi, Nobumichi ; Ikeda, Yasuo ; Kizaki, Masahiro. / Catechin, a green tea component, rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo. In: Haematologica. 2005 ; Vol. 90, No. 3. pp. 317-325.
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AU - Nakazato, Tomonori

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AU - Miyakawa, Yoshitaka

AU - Kinjo, Kentaro

AU - Yamada, Taketo

AU - Hozumi, Nobumichi

AU - Ikeda, Yasuo

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