Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract

Nancy L. Sheets, Bharesh K. Chauhan, Eric Wawrousek, J. Fielding Hejtmancik, Ales Cvekl, Marc Kantorow

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

PURPOSE. The Emory mouse is a well-characterized model for age-onset cataract. The purpose of the present study was to identify differentially expressed genes between pre- and postcataract Emory mouse lenses. METHODS. Eyes were extracted from Emory mice at 3 weeks (precataract) and 7.5 months (postcataract) of age, and lenses were dissected. Lens RNA was compared for gene expression differences by RT-PCR differential display, and transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The levels of two transcripts were further evaluated by RT-PCR in 3-week- and 7.5-month-old lenses and the remainder of the eye. The same transcripts were also measured in lenses from three non-Emory mouse strains (FVB/N, 129Sv, and CD1) ages 4 weeks to 11.5 months. RESULTS. Three transcripts were identified as exhibiting altered levels of gene expression between 3-week- and 7,5-month-old Emory mouse lenses. These encoded αA-crystallin (decreased), βA3/A1-crystallin (decreased), and adhesion-related kinase (ARK) receptor tyrosine kinase (increased). Decreased aA-crystallin and increased ARK expression were not detected in lenses isolated from three non-Emory mouse strains of similar age. Increased expression of ARK was not detected between 3-week- and 7.5-month-old Emory mouse eye nonlens tissues. CONCLUSIONS. The present data confirm that expression of the αA-crystallin gene is decreased in cataract in the Emory mouse lens relative to age-matched control lenses and they provide evidence for cataract- and lens-specific upregulation of the ARK receptor tyrosine kinase in the Emory mouse.

Original languageEnglish (US)
Pages (from-to)1870-1875
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume43
Issue number6
StatePublished - 2002
Externally publishedYes

Fingerprint

Receptor Protein-Tyrosine Kinases
Cataract
Lenses
Up-Regulation
Crystallins
Gene Expression
Phosphotransferases
Polymerase Chain Reaction
Crystalline Lens
Age of Onset
Genes
RNA

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Sheets, N. L., Chauhan, B. K., Wawrousek, E., Hejtmancik, J. F., Cvekl, A., & Kantorow, M. (2002). Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract. Investigative Ophthalmology and Visual Science, 43(6), 1870-1875.

Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract. / Sheets, Nancy L.; Chauhan, Bharesh K.; Wawrousek, Eric; Hejtmancik, J. Fielding; Cvekl, Ales; Kantorow, Marc.

In: Investigative Ophthalmology and Visual Science, Vol. 43, No. 6, 2002, p. 1870-1875.

Research output: Contribution to journalArticle

Sheets, NL, Chauhan, BK, Wawrousek, E, Hejtmancik, JF, Cvekl, A & Kantorow, M 2002, 'Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract', Investigative Ophthalmology and Visual Science, vol. 43, no. 6, pp. 1870-1875.
Sheets, Nancy L. ; Chauhan, Bharesh K. ; Wawrousek, Eric ; Hejtmancik, J. Fielding ; Cvekl, Ales ; Kantorow, Marc. / Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract. In: Investigative Ophthalmology and Visual Science. 2002 ; Vol. 43, No. 6. pp. 1870-1875.
@article{b0b5719e14e14c68b0a1034785535bce,
title = "Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract",
abstract = "PURPOSE. The Emory mouse is a well-characterized model for age-onset cataract. The purpose of the present study was to identify differentially expressed genes between pre- and postcataract Emory mouse lenses. METHODS. Eyes were extracted from Emory mice at 3 weeks (precataract) and 7.5 months (postcataract) of age, and lenses were dissected. Lens RNA was compared for gene expression differences by RT-PCR differential display, and transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The levels of two transcripts were further evaluated by RT-PCR in 3-week- and 7.5-month-old lenses and the remainder of the eye. The same transcripts were also measured in lenses from three non-Emory mouse strains (FVB/N, 129Sv, and CD1) ages 4 weeks to 11.5 months. RESULTS. Three transcripts were identified as exhibiting altered levels of gene expression between 3-week- and 7,5-month-old Emory mouse lenses. These encoded αA-crystallin (decreased), βA3/A1-crystallin (decreased), and adhesion-related kinase (ARK) receptor tyrosine kinase (increased). Decreased aA-crystallin and increased ARK expression were not detected in lenses isolated from three non-Emory mouse strains of similar age. Increased expression of ARK was not detected between 3-week- and 7.5-month-old Emory mouse eye nonlens tissues. CONCLUSIONS. The present data confirm that expression of the αA-crystallin gene is decreased in cataract in the Emory mouse lens relative to age-matched control lenses and they provide evidence for cataract- and lens-specific upregulation of the ARK receptor tyrosine kinase in the Emory mouse.",
author = "Sheets, {Nancy L.} and Chauhan, {Bharesh K.} and Eric Wawrousek and Hejtmancik, {J. Fielding} and Ales Cvekl and Marc Kantorow",
year = "2002",
language = "English (US)",
volume = "43",
pages = "1870--1875",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "6",

}

TY - JOUR

T1 - Cataract- and lens-specific upregulation of ARK receptor tyrosine kinase in emory mouse cataract

AU - Sheets, Nancy L.

AU - Chauhan, Bharesh K.

AU - Wawrousek, Eric

AU - Hejtmancik, J. Fielding

AU - Cvekl, Ales

AU - Kantorow, Marc

PY - 2002

Y1 - 2002

N2 - PURPOSE. The Emory mouse is a well-characterized model for age-onset cataract. The purpose of the present study was to identify differentially expressed genes between pre- and postcataract Emory mouse lenses. METHODS. Eyes were extracted from Emory mice at 3 weeks (precataract) and 7.5 months (postcataract) of age, and lenses were dissected. Lens RNA was compared for gene expression differences by RT-PCR differential display, and transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The levels of two transcripts were further evaluated by RT-PCR in 3-week- and 7.5-month-old lenses and the remainder of the eye. The same transcripts were also measured in lenses from three non-Emory mouse strains (FVB/N, 129Sv, and CD1) ages 4 weeks to 11.5 months. RESULTS. Three transcripts were identified as exhibiting altered levels of gene expression between 3-week- and 7,5-month-old Emory mouse lenses. These encoded αA-crystallin (decreased), βA3/A1-crystallin (decreased), and adhesion-related kinase (ARK) receptor tyrosine kinase (increased). Decreased aA-crystallin and increased ARK expression were not detected in lenses isolated from three non-Emory mouse strains of similar age. Increased expression of ARK was not detected between 3-week- and 7.5-month-old Emory mouse eye nonlens tissues. CONCLUSIONS. The present data confirm that expression of the αA-crystallin gene is decreased in cataract in the Emory mouse lens relative to age-matched control lenses and they provide evidence for cataract- and lens-specific upregulation of the ARK receptor tyrosine kinase in the Emory mouse.

AB - PURPOSE. The Emory mouse is a well-characterized model for age-onset cataract. The purpose of the present study was to identify differentially expressed genes between pre- and postcataract Emory mouse lenses. METHODS. Eyes were extracted from Emory mice at 3 weeks (precataract) and 7.5 months (postcataract) of age, and lenses were dissected. Lens RNA was compared for gene expression differences by RT-PCR differential display, and transcripts exhibiting altered levels of gene expression were cloned and identified by sequencing. The levels of two transcripts were further evaluated by RT-PCR in 3-week- and 7.5-month-old lenses and the remainder of the eye. The same transcripts were also measured in lenses from three non-Emory mouse strains (FVB/N, 129Sv, and CD1) ages 4 weeks to 11.5 months. RESULTS. Three transcripts were identified as exhibiting altered levels of gene expression between 3-week- and 7,5-month-old Emory mouse lenses. These encoded αA-crystallin (decreased), βA3/A1-crystallin (decreased), and adhesion-related kinase (ARK) receptor tyrosine kinase (increased). Decreased aA-crystallin and increased ARK expression were not detected in lenses isolated from three non-Emory mouse strains of similar age. Increased expression of ARK was not detected between 3-week- and 7.5-month-old Emory mouse eye nonlens tissues. CONCLUSIONS. The present data confirm that expression of the αA-crystallin gene is decreased in cataract in the Emory mouse lens relative to age-matched control lenses and they provide evidence for cataract- and lens-specific upregulation of the ARK receptor tyrosine kinase in the Emory mouse.

UR - http://www.scopus.com/inward/record.url?scp=0036272883&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036272883&partnerID=8YFLogxK

M3 - Article

VL - 43

SP - 1870

EP - 1875

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 6

ER -