Catalase-peroxidase of Caulobacter crescentus: Function and role in stationary-phase survival

Howard M. Steinman, Fareed Fareed, Laurie Weinstein

Research output: Contribution to journalArticle

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Abstract

Caulobacter crescentus is an obligate aerobe which is exposed to high concentrations of photosynthetic oxygen and low levels of nutrients in its aquatic environment. Physiological studies of oxidative and starvation stresses in C. crescentus were undertaken through a study of lacZ fusion and null mutant strains constructed from the cloned 5' end of katG, encoding a catalase-peroxidase. The katG gene was shown to be solely responsible for catalase and peroxidase activity in C. crescentus. Like the katG of Escherichia coli, C. crescentus katG is induced by hydrogen peroxide and is important in sustaining the exponential growth rate. However, dramatic differences are seen in growth stage induction. E. coli Kate catalase and KatG catalase-peroxidase activities are induced 15- to 20-fold during exponential growth and then approximately halved in the stationary phase. In contrast, C. crescentus KatG activity is constant throughout exponential growth and is induced 50-fold in the stationary phase. Moreover, the survival of a C. crescentus katG null mutant is reduced by more than 3 orders of magnitude after 24 h in stationary phase and more than 6 orders of magnitude after 48 h, a phenotype not seen for E. coli kate and katG null mutants. These results indicate a major role for C. crescentus catalase-peroxidase in stationary-phase survival and raise questions about whether the peroxidatic activity as well as the protective catalatic activity of the dual-function enzyme is important in the response to starvation stress.

Original languageEnglish (US)
Pages (from-to)6831-6836
Number of pages6
JournalJournal of Bacteriology
Volume179
Issue number21
StatePublished - Nov 1997

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Caulobacter crescentus
Catalase
Peroxidase
Growth
Escherichia coli
Starvation
Hydrogen Peroxide
Oxidative Stress
Oxygen
Phenotype
Food
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Catalase-peroxidase of Caulobacter crescentus : Function and role in stationary-phase survival. / Steinman, Howard M.; Fareed, Fareed; Weinstein, Laurie.

In: Journal of Bacteriology, Vol. 179, No. 21, 11.1997, p. 6831-6836.

Research output: Contribution to journalArticle

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