Catalase-peroxidase activity is decreased in a Caulobacter crescentus rho mutant

Valéria C S Italiani, Vânia S. Braz, Huifang Xiao, Howard M. Steinman, Marilis V. Marques

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A Caulobacter crescentus rhoTn5 mutant strain presenting a partially functional transcription termination factor Rho is highly sensitive to hydrogen peroxide in both exponential and stationary phases. The mutant was shown to be permanently under oxidative stress, based on fluorophore oxidation, and also to be sensitive to tert-butyl hydroperoxide and paraquat. However, the results showed that the activities of superoxide dismutases CuZnSOD and FeSOD and the alkylhydroperoxide reductase ahpC mRNA levels in the rho mutant were comparable to the wild-type control in the exponential and stationary phases. In contrast, the KatG catalase activity of the rho mutant strain was drastically decreased and did not show the expected increase in the stationary phase compared with the exponential phase. Transcription of the katG gene was increased in the rho mutant and the levels of the immunoreactive KatG protein do not differ considerably compared with the wild type in the stationary phase, suggesting that KatG activity is affected in a translational or a post-translational step.

Original languageEnglish (US)
Pages (from-to)48-54
Number of pages7
JournalFEMS Microbiology Letters
Volume303
Issue number1
DOIs
StatePublished - 2010

Fingerprint

Caulobacter crescentus
Peroxiredoxins
tert-Butylhydroperoxide
Paraquat
Catalase
Hydrogen Peroxide
Peroxidase
Superoxide Dismutase
Oxidative Stress
Transcription Factors
Messenger RNA
Genes
Proteins

Keywords

  • Catalase activity
  • Caulobacter crescentus
  • Rho mutant

ASJC Scopus subject areas

  • Microbiology
  • Genetics
  • Molecular Biology
  • Medicine(all)

Cite this

Catalase-peroxidase activity is decreased in a Caulobacter crescentus rho mutant. / Italiani, Valéria C S; Braz, Vânia S.; Xiao, Huifang; Steinman, Howard M.; Marques, Marilis V.

In: FEMS Microbiology Letters, Vol. 303, No. 1, 2010, p. 48-54.

Research output: Contribution to journalArticle

Italiani, Valéria C S ; Braz, Vânia S. ; Xiao, Huifang ; Steinman, Howard M. ; Marques, Marilis V. / Catalase-peroxidase activity is decreased in a Caulobacter crescentus rho mutant. In: FEMS Microbiology Letters. 2010 ; Vol. 303, No. 1. pp. 48-54.
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