Ca2+ binding, ATP-dependent Ca2+ transport, and total tissue Ca2+ in embryonic and adult avian dystrophic pectoralis

E. M. Ettienne, Robert H. Singer

Research output: Contribution to journalArticle

Abstract

Avian muscular dystrophy is an autosomal recessive genetic disease characterized by early hypertrophy and loss of function of the pectoralis major. The disease is progressive, ultimately resulting in atrophy and heavy lipid deposition. Previous investigators have noted a decrease in the ability of the dystrophic sarcoplasmic reticulum to concentrate Ca2+. More recently, other investigators have shown an abnormal calcium uptake in avian dystrophic sarcoplasmic reticulum. They indicated using freeze-fracture techniques, that a 90 θ particle of the vesicle membrane exhibited a decreased population and suggested that they might be the ATPase involved in calcium transport. The authors' studies confirm the earlier observations of a decreased rate of Ca2+ uptake and Ca2+ binding capacity of dystrophic fragmented sarcoplasmic reticulum vesicles which are isolated from both embryonic and adult pectoralis. These observations correlate in turn with 75% drop in the Ca:ATP transport efficiency of the dystrophic sarcoplasmic reticulum determined by measuring the rate of 32P(i) liberation from γ-ATP32 during active calcium transport by the isolated sarcoplasmic reticulum SR. In addition, the authors found a quantitative deficiency in a 65,000 dalton component of the dystrophic fragmented SR at the time of myoblast fusion by measuring 35S-Methionine incorporation into the SR, coupled to high resolution polyacrylamide gel electrophoresis and radioautography. Analysis of total tissue calcium by atomic absorption spectroscopy revealed a decrease in the total calcium content of dystrophic muscle.

Original languageEnglish (US)
Pages (from-to)195-210
Number of pages16
JournalJournal of Membrane Biology
Volume44
Issue number3-4
StatePublished - 1979
Externally publishedYes

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Sarcoplasmic Reticulum
Adenosine Triphosphate
Calcium
Research Personnel
Inborn Genetic Diseases
Muscular Dystrophies
Active Biological Transport
Myoblasts
Autoradiography
Methionine
Hypertrophy
Atrophy
Adenosine Triphosphatases
Polyacrylamide Gel Electrophoresis
Spectrum Analysis
Lipids
Muscles
Membranes
Population

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Physiology

Cite this

Ca2+ binding, ATP-dependent Ca2+ transport, and total tissue Ca2+ in embryonic and adult avian dystrophic pectoralis. / Ettienne, E. M.; Singer, Robert H.

In: Journal of Membrane Biology, Vol. 44, No. 3-4, 1979, p. 195-210.

Research output: Contribution to journalArticle

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