Abstract
Apoptosis can be measured by number of methods by taking advantage of the morphological, biochemical, and molecular changes undergoing in a cell during this process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fl uorochrome substrates, as well as employing the fl uorescein-labeled CaspaTag pan-caspase in situ detection kit.
Original language | English (US) |
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Journal | Methods in Molecular Biology |
Volume | 1219 |
DOIs | |
State | Published - 2015 |
Externally published | Yes |
Keywords
- Apoptosis
- Caspase-3
- Flow cytometry
- Immunohistochemistry
- Irradiation
- PARP-1
ASJC Scopus subject areas
- Molecular Biology
- Genetics