Caspase-3 Activation Is a Critical Determinant of Genotoxic Stress-Induced Apoptosis

Gaurav S. Choudhary, Sayer Al-harbi, Alexandru Almasan

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Apoptosis can be measured by number of methods by taking advantage of the morphological, biochemical, and molecular changes undergoing in a cell during this process. The best recognized biochemical hallmark of both early and late stages of apoptosis is the activation of cysteine proteases (caspases). Detection of active caspase-3 in cells and tissues is an important method for apoptosis induced by a wide variety of apoptotic signals. Most common assays for examining caspase-3 activation include immunostaining, immunoblotting for active caspase-3, colorimetric assays using fl uorochrome substrates, as well as employing the fl uorescein-labeled CaspaTag pan-caspase in situ detection kit.

Original languageEnglish (US)
JournalMethods in Molecular Biology
Volume1219
DOIs
StatePublished - 2015
Externally publishedYes

Keywords

  • Apoptosis
  • Caspase-3
  • Flow cytometry
  • Immunohistochemistry
  • Irradiation
  • PARP-1

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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