Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure

S. Lachtermacher, B. L B Esporcatte, F. Montalvão, P. C. Costa, D. C. Rodrigues, L. Belem, A. Rabischoffisky, H. C C Faria Neto, R. Vasconcellos, S. Iacobas, D. A. Iacobas, H. F R Dohmann, David C. Spray, R. C S Goldenberg, A. C. Campos-de-Carvalho

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

After myocardial infarction (MI), activation of the immune system and inflammatory mechanisms, among others, can lead to ventricular remodeling and heart failure (HF). The interaction between these systemic alterations and corresponding changes in the heart has not been extensively examined in the setting of chronic ischemia. The main purpose of this study was to investigate alterations in cardiac gene and systemic cytokine profile in mice with post-ischemic HF. Plasma was tested for IgM and IgG anti-heart reactive repertoire and inflammatory cytokines. Heart samples were assayed for gene expression by analyzing hybridization to AECOM 32k mouse microarrays. Ischemic HF significantly increased the levels of total serum IgM (by 5.2-fold) and total IgG (by 3.6-fold) associated with a relatively high content of anti-heart specificity. A comparable increase was observed in the levels of circulating pro-inflammatory cytokines such as IL-1β (3.8X) and TNF-α (6.0X). IFN-γ was also increased by 3.1-fold in the MI group. However, IL-4 and IL-10 were not significantly different between the MI and sham-operated groups. Chemokines such as MCP-1 and IL-8 were 1.4- and 13-fold increased, respectively, in the plasma of infarcted mice. We identified 2079 well annotated unigenes that were significantly regulated by post-ischemic HF. Complement activation and immune response were among the most up-regulated processes. Interestingly, 21 of the 101 quantified unigenes involved in the inflammatory response were significantly up-regulated and none were down-regulated. These data indicate that post-ischemic heart remodeling is accompanied by immune-mediated mechanisms that act both systemically and locally.

Original languageEnglish (US)
Pages (from-to)377-389
Number of pages13
JournalBrazilian Journal of Medical and Biological Research
Volume43
Issue number4
StatePublished - Apr 2010

Fingerprint

Gene expression
Heart Failure
Cytokines
Gene Expression
Myocardial Infarction
Ventricular Remodeling
Complement Activation
Interleukin-8
Interleukin-1
Chemokines
Interleukin-4
Interleukin-10
Immunoglobulin M
Immune System
Chemical activation
Ischemia
Immunoglobulin G
Plasmas
Immune system
Microarrays

Keywords

  • Anti-heart antibodies
  • Cytokines
  • Experimental post-ischemic heart failure
  • Immunoarray
  • RNAm - Microarray

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Physiology
  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Lachtermacher, S., Esporcatte, B. L. B., Montalvão, F., Costa, P. C., Rodrigues, D. C., Belem, L., ... Campos-de-Carvalho, A. C. (2010). Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. Brazilian Journal of Medical and Biological Research, 43(4), 377-389.

Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. / Lachtermacher, S.; Esporcatte, B. L B; Montalvão, F.; Costa, P. C.; Rodrigues, D. C.; Belem, L.; Rabischoffisky, A.; Faria Neto, H. C C; Vasconcellos, R.; Iacobas, S.; Iacobas, D. A.; Dohmann, H. F R; Spray, David C.; Goldenberg, R. C S; Campos-de-Carvalho, A. C.

In: Brazilian Journal of Medical and Biological Research, Vol. 43, No. 4, 04.2010, p. 377-389.

Research output: Contribution to journalArticle

Lachtermacher, S, Esporcatte, BLB, Montalvão, F, Costa, PC, Rodrigues, DC, Belem, L, Rabischoffisky, A, Faria Neto, HCC, Vasconcellos, R, Iacobas, S, Iacobas, DA, Dohmann, HFR, Spray, DC, Goldenberg, RCS & Campos-de-Carvalho, AC 2010, 'Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure', Brazilian Journal of Medical and Biological Research, vol. 43, no. 4, pp. 377-389.
Lachtermacher, S. ; Esporcatte, B. L B ; Montalvão, F. ; Costa, P. C. ; Rodrigues, D. C. ; Belem, L. ; Rabischoffisky, A. ; Faria Neto, H. C C ; Vasconcellos, R. ; Iacobas, S. ; Iacobas, D. A. ; Dohmann, H. F R ; Spray, David C. ; Goldenberg, R. C S ; Campos-de-Carvalho, A. C. / Cardiac gene expression and systemic cytokine profile are complementary in a murine model of post-ischemic heart failure. In: Brazilian Journal of Medical and Biological Research. 2010 ; Vol. 43, No. 4. pp. 377-389.
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