We investigated the capillary differentiation of endothelial cells cultured on microgrooved polydimethylsiloxane (PDMS) substrates coated with fibronectin. Bovine aortic endothelial (BAE) cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) on PDMS substrates produced photolithographically. Phase contrast images of BAE cells were taken to assess the overall morphology of cells. Confocal microscopy was used to examine the existence of lumen inside cord-like and tube-like structures developed at different stages of cell culture. Herein we show for the first time that microgrooved PDMS substrates of appropriate dimensions coated with fibronectin are capable of inducing capillary differentiation of endothelial cells in the absence of angiogenic growth factors.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films