Candida parapsilosis fat storage-inducing transmembrane (FIT) protein 2 regulates lipid droplet formation and impacts virulence

Long N. Nguyen, Zsuzsanna Hamari, Bert Kadereit, David Trofa, Mariangela Agovino, Luis R. Martinez, Attila Gacser, David L. Silver, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Neutral lipid storage in lipid droplets (LDs) is a conserved process across diverse species. Although significant attention has focused on LDs in the biology of obesity, diabetes, and atherosclerosis, there is limited information on the role of LDs in pathogenic fungi. We have disrupted the Fat storage-Inducing Transmembrane (FIT) protein 2 genes of the emerging pathogenic fungus Candida parapsilosis and demonstrated that LD formation is significantly reduced in the mutant cells. Disruption of FIT2 genes also reduced accumulation of triacylglycerols. The production of other lipids such as phospholipids and steryl esters were also affected in the mutant strain. Inhibition of de novo fatty acid biosynthesis by triclosan in the FIT2 disruptants reduced fungal growth in rich medium YPD, indicating that TAGs or fatty acids from the LDs could be important for cell proliferation. FIT2 disruption was associated with enhanced sensitivity to oxidative stress. Furthermore, we showed that FIT2 deletion yeast cells were significantly attenuated in murine infection models, suggesting an involvement of LDs in the pathobiology of the fungus.

Original languageEnglish (US)
Pages (from-to)663-672
Number of pages10
JournalMicrobes and Infection
Volume13
Issue number7
DOIs
StatePublished - Jul 2011

Fingerprint

Candida
Virulence
Fats
Proteins
Fungi
Fatty Acids
Triclosan
Lipids
Lipid Droplets
Phospholipids
Atherosclerosis
Esters
Triglycerides
Oxidative Stress
Obesity
Yeasts
Cell Proliferation
Growth
Infection
Genes

Keywords

  • Candida
  • Fatty acid synthesis
  • Lipid droplet
  • Pathogenesis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Candida parapsilosis fat storage-inducing transmembrane (FIT) protein 2 regulates lipid droplet formation and impacts virulence. / Nguyen, Long N.; Hamari, Zsuzsanna; Kadereit, Bert; Trofa, David; Agovino, Mariangela; Martinez, Luis R.; Gacser, Attila; Silver, David L.; Nosanchuk, Joshua D.

In: Microbes and Infection, Vol. 13, No. 7, 07.2011, p. 663-672.

Research output: Contribution to journalArticle

Nguyen, Long N. ; Hamari, Zsuzsanna ; Kadereit, Bert ; Trofa, David ; Agovino, Mariangela ; Martinez, Luis R. ; Gacser, Attila ; Silver, David L. ; Nosanchuk, Joshua D. / Candida parapsilosis fat storage-inducing transmembrane (FIT) protein 2 regulates lipid droplet formation and impacts virulence. In: Microbes and Infection. 2011 ; Vol. 13, No. 7. pp. 663-672.
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