Candida glabrata produces a melanin-like pigment that protects against stress conditions encountered during parasitism

Rodrigo Almeida-Paes, Maria H.G. Figueiredo-Carvalho, Leandro B.R. Da Silva, Gary Gerfen, G. R. De S Araújo, Susana Frases, Rosely M. Zancopé-Oliveira, Joshua D. Nosanchuk

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: Melanin has been linked to pathogenesis in several fungi. They often produce melanin-like pigments in the presence of L-dihydroxyphenylalanine (L-DOPA), but this is poorly studied in Candida glabrata. Methods & materials: C. glabrata was grown in minimal medium with or without L-DOPA supplementation and submitted to a chemical treatment with denaturant and hot acid. Results: C. glabrata turned black when grown in the presence of L-DOPA, whereas cells grown without L-DOPA supplementation remained white. Biophysical properties demonstrated that the pigment was melanin. Melanized C. glabrata cells were effectively protected from azoles and amphotericin B, incubation at 42°C and macrophage killing. Conclusion: In the presence of L-DOPA, C. glabrata produces melanin, increases antifungal resistance and enhances host survival.

Original languageEnglish (US)
Pages (from-to)509-520
Number of pages12
JournalFuture Microbiology
Volume16
Issue number7
DOIs
StatePublished - May 2021
Externally publishedYes

Keywords

  • Candida glabrata
  • L-DOPA
  • melanin
  • survival
  • virulence

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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