TY - JOUR
T1 - Candida albicans and Candida parapsilosis induce different T-cell responses in human peripheral blood mononuclear cells
AU - Tóth, Adél
AU - Csonka, Katalin
AU - Jacobs, Cor
AU - Vágvölgyi, Csaba
AU - Nosanchuk, Joshua D.
AU - Netea, Mihai G.
AU - Gácser, Attila
N1 - Funding Information:
Financial support. This work was supported by the Federation of European Microbiological Societies (research fellowship to A. T.), OTKA (NF 84006 and ERA-Net Pathogenomics NN100374 to A. G.), EMBO (installation grant 1813 to A. G.), the Hungarian Scientific Research Fund (NF 84006 and ERA-Net Pathogenomics NN100374 to A. T. and A. G.), the National Institutes of Health (grant AI52733 to J. N.), the Irma T. Hirschl/Monique Weill-Caulier Trust (research award to J. N.), and the Netherlands Organization for Scientific Research (Vici grant to M. G. N.). Potential conflicts of interest. All authors: No reported conflicts.
PY - 2013/8/15
Y1 - 2013/8/15
N2 - Candida parapsilosis is the third most frequent cause of candidemia. Despite its clinical importance, little is known about the human immunological response to C. parapsilosis. In this study, we compared the cytokine responses evoked by Candida albicans and C. parapsilosis. C. parapsilosis-stimulated human peripheral blood mononuclear cells (PBMCs) produced similar quantities of tumor necrosis factor α and interleukin 6 and slightly lower amounts of interleukin 1β, compared with C. albicans-stimulated cells. PBMCs stimulated with C. parapsilosis displayed a skewed T-helper cell response, producing more interleukin 10 and less interferon γ than cells stimulated with C. albicans. Notably, C. parapsilosis induced much less interleukin 17 and interleukin 22 production as compared to C. albicans. Inhibition of the 3 classical mitogen-activated protein kinases (p38 kinase, ERK, and JNK) revealed kinase-dependent differences in reductions in cytokine production by the 2 Candida species. Decreased cytokine production after inhibition of dectin 1 revealed that this receptor plays a major role in the recognition of both C. albicans and C. parapsilosis. These data improve understanding of the immune response triggered by C. parapsilosis, a first step for the future design of immunotherapeutic strategies for these infections.
AB - Candida parapsilosis is the third most frequent cause of candidemia. Despite its clinical importance, little is known about the human immunological response to C. parapsilosis. In this study, we compared the cytokine responses evoked by Candida albicans and C. parapsilosis. C. parapsilosis-stimulated human peripheral blood mononuclear cells (PBMCs) produced similar quantities of tumor necrosis factor α and interleukin 6 and slightly lower amounts of interleukin 1β, compared with C. albicans-stimulated cells. PBMCs stimulated with C. parapsilosis displayed a skewed T-helper cell response, producing more interleukin 10 and less interferon γ than cells stimulated with C. albicans. Notably, C. parapsilosis induced much less interleukin 17 and interleukin 22 production as compared to C. albicans. Inhibition of the 3 classical mitogen-activated protein kinases (p38 kinase, ERK, and JNK) revealed kinase-dependent differences in reductions in cytokine production by the 2 Candida species. Decreased cytokine production after inhibition of dectin 1 revealed that this receptor plays a major role in the recognition of both C. albicans and C. parapsilosis. These data improve understanding of the immune response triggered by C. parapsilosis, a first step for the future design of immunotherapeutic strategies for these infections.
KW - Candida parapsilosis
KW - Human PBMC
KW - T cell response
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U2 - 10.1093/infdis/jit188
DO - 10.1093/infdis/jit188
M3 - Article
C2 - 23661798
AN - SCOPUS:84880947536
SN - 0022-1899
VL - 208
SP - 690
EP - 698
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -