Can your protein be sumoylated? A quick summary and important tips to study SUMO-modified proteins

Yuxuan Xiao, Daniel Pollack, Edward Nieves, Aby Winchell, Myrasol Callaway, Margarita Vigodner

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A diverse set of SUMO target proteins has been identified. Therefore, there is a growing interest in studying sumoylation and SUMO interactions in cells. When the sumoylation of a protein or a SUMO interaction is suspected, a standard co-immunoprecipitation analysis using anti-SUMO and anti-target protein antibody is usually performed as a first step. However, the identification of endogenous sumoylated proteins is challenging because of the activity of isopeptidases, and often only a small fraction of a target protein is sumoylated at a given time. Here, we briefly summarize several important steps to ensure a successful co-immunoprecipitation analysis to detect possible sumoylation.

Original languageEnglish (US)
Pages (from-to)95-97
Number of pages3
JournalAnalytical Biochemistry
Volume477
DOIs
StatePublished - May 15 2015

Keywords

  • Co-immunoprecipitation
  • In vitro sumoylation
  • Isopeptidases
  • Mass spectrometry
  • SUMO
  • Sumoylation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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