Can the stem cell mobilization technique influence CD34+ cell collection efficiency of leukapheresis procedures in patients with hematologic malignancies?

A. Gidron, A. Verma, M. Doyle, L. Boggio, A. Evens, L. Gordon, S. Singhal, M. Tallman, S. Wlliams, J. Winter, J. Mehta

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

A total of 415 leukaphereses in 201 patients stimulated with growth factor (GF; n = 119) or chemotherapy-GF (n = 296) were studied to determine CD34 + cell collection efficiency (CE). The pre-apheresis leukocyte count was 1-93 × 109/l (median 20), and peripheral blood CD34 count (PBCD34) was 1-1104/μl (median 19). The total number of CD34+ cells collected was 4-6531 × 106 (median 151); corresponding to 0.1-111.4 × 106 (median 2.3) per kg. There was strong correlation between PBCD34 and the number of CD34+ cells collected (r = 0.9; P < 0.0001). CE was 7-145% (median 46). On multiple regression analysis, a higher leukocyte count (P < 0.0001) was the most important predictor of lower CE. CE with leukocytes < 20 was 7-145% (median 53%) compared to 10-132% (median 40%) with leukocyte ≥ 20 (P < 0.0001). In all, 61% of the apheresis procedures performed after chemotherapy-GF occurred when leukocytes were < 20 compared to 21% of those performed after GF alone (P < 0.0001). We conclude that mobilizing patients with the combination of chemotherapy and GF rather than GF alone leads to leukapheresis being performed when the leukocyte count is low - in a range that results in optimum CD34+ cell CE. Autologous stem cells should be mobilized with chemotherapy-GF rather than GF alone whenever possible.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalBone Marrow Transplantation
Volume35
Issue number3
DOIs
StatePublished - Feb 1 2005
Externally publishedYes

Keywords

  • CD34+ cells
  • Cell separator
  • Collection efficiency
  • Leukapheresis

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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