TY - JOUR
T1 - Can oxygen tension contribute to an abnormal placental cytokine milieu?
AU - Peltier, Morgan R.
AU - Gurzenda, Ellen M.
AU - Murthy, Amitasrigowri
AU - Chawala, Kiranpreet
AU - Lerner, Veronica
AU - Kharode, Ishita
AU - Arita, Yuko
AU - Rhodes, Adam
AU - Maari, Nisreen
AU - Moawad, Andrew
AU - Hanna, Nazeeh
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/10
Y1 - 2011/10
N2 - The aim of this study was to determine whether culturing human placental explants under different oxygen tensions will alter expression of pro- and anti-inflammatory mediators. Methods Placental explant cultures from second-trimester, elective, terminations-of-pregnancy were incubated under 21, 5, or 1% O 2 concentrations for 24hr in the presence or absence of IL-10. Cytokine concentrations in the conditioned medium were quantified by immunoassay. Results Culture of placental explants under 21, 5, or 1% O 2 concentrations produced hyperoxic (143±1.6mmHg), normoxic (37±1.6mmHg), and hypoxic (18.2±1.6mmHg) pO 2 levels for the maternal-fetal interface in the medium. Oxygen tension had profound effects on basal placental cytokine levels as well as on IL-10-stimulated cytokine production. IL-1β and TNF-α, but not IFN-γ production, was reduced by 21% O 2. Moreover, 21% O 2 levels increased the anti-inflammatory cytokines IL-10 and IL-13 while 1% O 2 tended to decrease the production of these cytokines. Conclusions Five percent- O 2 incubation more accurately represents in vivo pO 2 conditions at the maternal-fetal interface. Routine culture of placental explants in room air produces a superphysiologic oxygen tension that tended to increase the production of anti-inflammatory and decrease the production of pro-inflammatory cytokines. In addition, low pO 2 may reduce responsiveness of the placenta to the anti-inflammatory actions of IL-10.
AB - The aim of this study was to determine whether culturing human placental explants under different oxygen tensions will alter expression of pro- and anti-inflammatory mediators. Methods Placental explant cultures from second-trimester, elective, terminations-of-pregnancy were incubated under 21, 5, or 1% O 2 concentrations for 24hr in the presence or absence of IL-10. Cytokine concentrations in the conditioned medium were quantified by immunoassay. Results Culture of placental explants under 21, 5, or 1% O 2 concentrations produced hyperoxic (143±1.6mmHg), normoxic (37±1.6mmHg), and hypoxic (18.2±1.6mmHg) pO 2 levels for the maternal-fetal interface in the medium. Oxygen tension had profound effects on basal placental cytokine levels as well as on IL-10-stimulated cytokine production. IL-1β and TNF-α, but not IFN-γ production, was reduced by 21% O 2. Moreover, 21% O 2 levels increased the anti-inflammatory cytokines IL-10 and IL-13 while 1% O 2 tended to decrease the production of these cytokines. Conclusions Five percent- O 2 incubation more accurately represents in vivo pO 2 conditions at the maternal-fetal interface. Routine culture of placental explants in room air produces a superphysiologic oxygen tension that tended to increase the production of anti-inflammatory and decrease the production of pro-inflammatory cytokines. In addition, low pO 2 may reduce responsiveness of the placenta to the anti-inflammatory actions of IL-10.
KW - Cytokine
KW - Hyperoxia
KW - Hypoxia
KW - Inflammation
KW - Oxygen tension
KW - Placenta
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U2 - 10.1111/j.1600-0897.2011.00998.x
DO - 10.1111/j.1600-0897.2011.00998.x
M3 - Article
C2 - 21481060
AN - SCOPUS:80052562487
SN - 1046-7408
VL - 66
SP - 279
EP - 285
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 4
ER -