Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice

Farzin Yaghmaie, Omar Saeed, Steven A. Garan, Warren Freitag, Paola S. Timiras, Hal Sternberg

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Life-long calorie restriction (CR) remains the most robust and reliable means of extending life span in mammals. Among the several theories to explain CR actions, one variant of the neuroendocrine theories of aging postulates that changing hypothalamic sensitivity to endocrine feedback is the clock that times phenotypic change over the life span. If the feedback sensitivity hypothesis is correct, CR animals should display a significantly different pattern of hormone-sensitive cell density and distribution in the hypothalamus. Of the many endocrine signal receptors that may be involved in maintaining hypothalamic feedback sensitivity, our study has selected to begin mapping those for estrogen (E). Altered hypothalamic sensitivity to E is known to schedule reproductive maturation and influence reproductive senescence. Taking estrogen receptor-alpha (ERα) immunoreactivity as an index of sensitivity to E, we counted ERα immunoreactive and non-immunoreactive cells in the pre-optic hypothalamus of young (6 weeks), ad-libitum (Old-AL) fed old (22 months), and calorie restricted (Old-CR) old (22 months) female B6D2F1 mice. An automated imaging microscopy system (AIMS) was used to generate cell counts for each sampled section of pre-optic hypothalamus. Results show a 38% reduction in ERα immunoreactive cells and an 18% reduction in total cell numbers in AL-old mice in comparison to young mice. However, CR mice only show a 19% reduction in ERα immunoreactive cells and a 13% reduction in total cell numbers in comparison to young mice. This indicates CR prevents age-related cell loss and maintains estrogen sensitivity in the pre-optic hypothalamus of old female B6D2F1 mice.

Original languageEnglish (US)
Pages (from-to)197-203
Number of pages7
JournalNeuroendocrinology Letters
Volume26
Issue number3
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Caloric Restriction
Estrogen Receptor alpha
Hypothalamus
Cell Count
Estrogens
Mammals
Microscopy
Appointments and Schedules
Hormones

Keywords

  • Aging
  • Caloric restriction
  • Estrogen receptor-alpha
  • Hypothalamus

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice. / Yaghmaie, Farzin; Saeed, Omar; Garan, Steven A.; Freitag, Warren; Timiras, Paola S.; Sternberg, Hal.

In: Neuroendocrinology Letters, Vol. 26, No. 3, 06.2005, p. 197-203.

Research output: Contribution to journalArticle

Yaghmaie, Farzin ; Saeed, Omar ; Garan, Steven A. ; Freitag, Warren ; Timiras, Paola S. ; Sternberg, Hal. / Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice. In: Neuroendocrinology Letters. 2005 ; Vol. 26, No. 3. pp. 197-203.
@article{9abf5b38f7b64d43ae9cdd6dccdefab6,
title = "Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice",
abstract = "Life-long calorie restriction (CR) remains the most robust and reliable means of extending life span in mammals. Among the several theories to explain CR actions, one variant of the neuroendocrine theories of aging postulates that changing hypothalamic sensitivity to endocrine feedback is the clock that times phenotypic change over the life span. If the feedback sensitivity hypothesis is correct, CR animals should display a significantly different pattern of hormone-sensitive cell density and distribution in the hypothalamus. Of the many endocrine signal receptors that may be involved in maintaining hypothalamic feedback sensitivity, our study has selected to begin mapping those for estrogen (E). Altered hypothalamic sensitivity to E is known to schedule reproductive maturation and influence reproductive senescence. Taking estrogen receptor-alpha (ERα) immunoreactivity as an index of sensitivity to E, we counted ERα immunoreactive and non-immunoreactive cells in the pre-optic hypothalamus of young (6 weeks), ad-libitum (Old-AL) fed old (22 months), and calorie restricted (Old-CR) old (22 months) female B6D2F1 mice. An automated imaging microscopy system (AIMS) was used to generate cell counts for each sampled section of pre-optic hypothalamus. Results show a 38{\%} reduction in ERα immunoreactive cells and an 18{\%} reduction in total cell numbers in AL-old mice in comparison to young mice. However, CR mice only show a 19{\%} reduction in ERα immunoreactive cells and a 13{\%} reduction in total cell numbers in comparison to young mice. This indicates CR prevents age-related cell loss and maintains estrogen sensitivity in the pre-optic hypothalamus of old female B6D2F1 mice.",
keywords = "Aging, Caloric restriction, Estrogen receptor-alpha, Hypothalamus",
author = "Farzin Yaghmaie and Omar Saeed and Garan, {Steven A.} and Warren Freitag and Timiras, {Paola S.} and Hal Sternberg",
year = "2005",
month = "6",
language = "English (US)",
volume = "26",
pages = "197--203",
journal = "Neuroendocrinology Letters",
issn = "0172-780X",
publisher = "Maghira and Maas Publications",
number = "3",

}

TY - JOUR

T1 - Caloric restriction reduces cell loss and maintains estrogen receptor-alpha immunoreactivity in the pre-optic hypothalamus of female B6D2F1 mice

AU - Yaghmaie, Farzin

AU - Saeed, Omar

AU - Garan, Steven A.

AU - Freitag, Warren

AU - Timiras, Paola S.

AU - Sternberg, Hal

PY - 2005/6

Y1 - 2005/6

N2 - Life-long calorie restriction (CR) remains the most robust and reliable means of extending life span in mammals. Among the several theories to explain CR actions, one variant of the neuroendocrine theories of aging postulates that changing hypothalamic sensitivity to endocrine feedback is the clock that times phenotypic change over the life span. If the feedback sensitivity hypothesis is correct, CR animals should display a significantly different pattern of hormone-sensitive cell density and distribution in the hypothalamus. Of the many endocrine signal receptors that may be involved in maintaining hypothalamic feedback sensitivity, our study has selected to begin mapping those for estrogen (E). Altered hypothalamic sensitivity to E is known to schedule reproductive maturation and influence reproductive senescence. Taking estrogen receptor-alpha (ERα) immunoreactivity as an index of sensitivity to E, we counted ERα immunoreactive and non-immunoreactive cells in the pre-optic hypothalamus of young (6 weeks), ad-libitum (Old-AL) fed old (22 months), and calorie restricted (Old-CR) old (22 months) female B6D2F1 mice. An automated imaging microscopy system (AIMS) was used to generate cell counts for each sampled section of pre-optic hypothalamus. Results show a 38% reduction in ERα immunoreactive cells and an 18% reduction in total cell numbers in AL-old mice in comparison to young mice. However, CR mice only show a 19% reduction in ERα immunoreactive cells and a 13% reduction in total cell numbers in comparison to young mice. This indicates CR prevents age-related cell loss and maintains estrogen sensitivity in the pre-optic hypothalamus of old female B6D2F1 mice.

AB - Life-long calorie restriction (CR) remains the most robust and reliable means of extending life span in mammals. Among the several theories to explain CR actions, one variant of the neuroendocrine theories of aging postulates that changing hypothalamic sensitivity to endocrine feedback is the clock that times phenotypic change over the life span. If the feedback sensitivity hypothesis is correct, CR animals should display a significantly different pattern of hormone-sensitive cell density and distribution in the hypothalamus. Of the many endocrine signal receptors that may be involved in maintaining hypothalamic feedback sensitivity, our study has selected to begin mapping those for estrogen (E). Altered hypothalamic sensitivity to E is known to schedule reproductive maturation and influence reproductive senescence. Taking estrogen receptor-alpha (ERα) immunoreactivity as an index of sensitivity to E, we counted ERα immunoreactive and non-immunoreactive cells in the pre-optic hypothalamus of young (6 weeks), ad-libitum (Old-AL) fed old (22 months), and calorie restricted (Old-CR) old (22 months) female B6D2F1 mice. An automated imaging microscopy system (AIMS) was used to generate cell counts for each sampled section of pre-optic hypothalamus. Results show a 38% reduction in ERα immunoreactive cells and an 18% reduction in total cell numbers in AL-old mice in comparison to young mice. However, CR mice only show a 19% reduction in ERα immunoreactive cells and a 13% reduction in total cell numbers in comparison to young mice. This indicates CR prevents age-related cell loss and maintains estrogen sensitivity in the pre-optic hypothalamus of old female B6D2F1 mice.

KW - Aging

KW - Caloric restriction

KW - Estrogen receptor-alpha

KW - Hypothalamus

UR - http://www.scopus.com/inward/record.url?scp=22144499238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22144499238&partnerID=8YFLogxK

M3 - Article

C2 - 15990721

AN - SCOPUS:22144499238

VL - 26

SP - 197

EP - 203

JO - Neuroendocrinology Letters

JF - Neuroendocrinology Letters

SN - 0172-780X

IS - 3

ER -