Calcium-activated potassium channels are selectively coupled to P/Q-type calcium channels in cerebellar Purkinje neurons

Mary D. Womack, Carolyn Chevez, Kamran Khodakhah

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Cerebellar Purkinje neurons fire spontaneously in the absence of synaptic transmission. P/Q-type voltage-gated calcium channels and calcium-activated potassium channels are required for normal spontaneous activity. Blocking P/Q-type calcium channels paradoxically mimics the effects of blocking calcium-activated potassium channels. Thus, an important function of the P/Q-type calcium channels is to provide calcium for activation of calcium-activated potassium channels. Purkinje neurons express several classes of voltage-gated calcium channels, and the P/Q- and T-type channels make comparable contributions to total calcium entry after an action potential. Here we demonstrate that calcium-activated potassium channels are activated exclusively by calcium entering through P/Q-type voltage-gated calcium channels. This selective coupling is maintained even when calcium flux through voltage-gated channels is increased by increasing the extracellular calcium concentration. Small decreases in P/Q current density are likely to alter spontaneous activity of Purkinje neurons via decreased recruitment of calcium-activated potassium channels. In both human and murine animal models, mutations that decrease P/Q current density in Purkinje neurons also cause cerebellar ataxia. Alterations in the spontaneous activity of Purkinje neurons may be an important contributing factor to the ataxia in these subjects.

Original languageEnglish (US)
Pages (from-to)8818-8822
Number of pages5
JournalJournal of Neuroscience
Volume24
Issue number40
DOIs
StatePublished - Oct 6 2004

Fingerprint

Q-Type Calcium Channels
P-Type Calcium Channels
Calcium-Activated Potassium Channels
Purkinje Cells
Calcium
Calcium Channels
Cerebellar Ataxia
Ataxia
Synaptic Transmission
Action Potentials
Animal Models
Mutation

Keywords

  • Action potential
  • AHP
  • Calcium channel
  • Calcium-activated potassium channel
  • Cerebellum
  • Coupling
  • Purkinje cell
  • Spontaneous firing

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Calcium-activated potassium channels are selectively coupled to P/Q-type calcium channels in cerebellar Purkinje neurons. / Womack, Mary D.; Chevez, Carolyn; Khodakhah, Kamran.

In: Journal of Neuroscience, Vol. 24, No. 40, 06.10.2004, p. 8818-8822.

Research output: Contribution to journalArticle

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