Caenorhabditis elegans TRPV ion channel regulates 5HT biosynthesis in chemosensory neurons

Shenyuan Zhang, Irina Sokolchik, Gabriela Blanco, Ji Ying Sze

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Serotonin (5HT) is a pivotal signaling molecule that modulates behavioral and endocrine responses to diverse chemical and physical stimuli. We report cell-specific regulation of 5HT biosynthesis by transient receptor potential V (TRPV) ion channels in C. elegans. Mutations in the TRPV genes osm-9 or ocr-2 dramatically downregulate the expression of the gene encoding the 5HT synthesis enzyme tryptophan hydroxylase (tph-1) in the serotonergic chemosensory neurons ADF, but neither the mutation nor the double mutation of both channel genes affects other types of serotonergic neurons. The TRPV genes are expressed in the ADF neurons but not in other serotonergic neurons, and act cell-autonomously to regulate a neuron-specific transcription program. Whereas in olfactory neurons OSM-9 and OCR-2 function is dependent on ODR-3 Gα, the activity of ODR-3 or two other Gα proteins expressed in the ADF neurons is not required for upregulating tph-1 expression, thus the TRPV ion channels in different neurons may be regulated by different mechanisms. A gain-of-function mutation in CaMKII UNC-43 partially suppresses the downregulation of tph-1 in the TRPV mutants, thus CaMKII may be an effector of the TRPV signaling. Mutations in the TRPV genes cause worms developmentally arrest at the Dauer stage. This developmental defect is due in part to reduced 5HT inputs into daf-2/insulin neuroendocrine signaling.

Original languageEnglish (US)
Pages (from-to)1629-1638
Number of pages10
JournalDevelopment
Volume131
Issue number7
DOIs
StatePublished - Apr 2004
Externally publishedYes

Fingerprint

Caenorhabditis elegans
Ion Channels
Serotonergic Neurons
Neurons
Mutation
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Genes
Down-Regulation
Tryptophan Hydroxylase
GTP-Binding Proteins
Serotonin
Insulin
Gene Expression
Enzymes

Keywords

  • C. elegans
  • Metabolic control
  • Serotonin biosynthesis
  • Transcription
  • TRPV ion channels

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Caenorhabditis elegans TRPV ion channel regulates 5HT biosynthesis in chemosensory neurons. / Zhang, Shenyuan; Sokolchik, Irina; Blanco, Gabriela; Sze, Ji Ying.

In: Development, Vol. 131, No. 7, 04.2004, p. 1629-1638.

Research output: Contribution to journalArticle

Zhang, Shenyuan ; Sokolchik, Irina ; Blanco, Gabriela ; Sze, Ji Ying. / Caenorhabditis elegans TRPV ion channel regulates 5HT biosynthesis in chemosensory neurons. In: Development. 2004 ; Vol. 131, No. 7. pp. 1629-1638.
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