C nmr assignments of the protonated carbonof [d(Tagcgcta)]2 by twodimensional proton-detected heteronuclear correlation

Teven R. Laplante, Joseph Ashcroft, David Cowbum, George C. Levy, Philip N. Borer

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The resonances of the protonated carbons of [d(TAGCGCTA)]2 have been assigned by the two-dimensional proton-detected double-quantum heteronuclear correlation experiment ([1Hl3C]-DQCOSY).13C-coupled andl3C-decoupled versions of the experiment were used. The assignment method is discussed in detail. The deoxyribose cross peaks segregate into five well-resolved regions, and the base cross peaks have distinct features that are helpful for assignments. The cross peaks from the ‘H-13C pairs at the Cyd5, Ado2 and ThdCH3 base positions fall in separate regions of the spectrum from each other; they also are resolved from the closely spaced Ado8, Guo8, Cyd6 and Thd6. Additional parameters for distinction of the base signals are their differing J-coupling values and long-range coupling patterns.

Original languageEnglish (US)
Pages (from-to)1089-1100
Number of pages12
JournalJournal of Biomolecular Structure and Dynamics
Volume5
Issue number5
DOIs
StatePublished - Apr 1988
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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