c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function

S. W.Grace Cheng, Kelvin P. Davies, Eric Yung, Ralph J. Beltran, Jin Yu, Ganjam V. Kalpana

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Abstract

Chromatin organization plays a key role in the regulation of gene expression. The evolutionarily conserved SWI/SNF complex is one of several multiprotein complexes that activate transcription by remodelling chromatin in an ATP-dependent manner. SWI2/SNF2 is an ATPase whose homologues, BRG1 and hBRM, mediate cell-cycle arrest; the SNF5 homologue, INI1/hSNF5, appears to be a turnout suppressor. A search for INI1-interacting proteins using the two-hybrid system led to the isolation of c-MYC, a transactivator. The c- MYC-INI1 interaction was observed both in vitro and in vivo. The c-MYC basic helix-loop-helix (bHLH) and leucine zipper (Zip) domains and the INI1 repeat 1 (Rpt1) region were required for this interaction, c-MYC-mediated transactivation was inhibited by a deletion fragment of INI1 and the ATPase mutant of BRG1/hSNF2 in a dominant-negative manner contingent upon the presence of the c-MYC bHLH-Zip domain. Our results suggest that the SWI/SNF complex is necessary for c-MYC-mediated transactivation and that the c-MYC- INI1 interaction helps recruit the complex.

Original languageEnglish (US)
Pages (from-to)102-105
Number of pages4
JournalNature Genetics
Volume22
Issue number1
DOIs
StatePublished - May 1 1999

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ASJC Scopus subject areas

  • Genetics

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