c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function

S. W Grace Cheng, Kelvin Davies, Eric Yung, Ralph J. Beltran, Jin Yu, Ganjam V. Kalpana

Research output: Contribution to journalArticle

287 Citations (Scopus)

Abstract

Chromatin organization plays a key role in the regulation of gene expression. The evolutionarily conserved SWI/SNF complex is one of several multiprotein complexes that activate transcription by remodelling chromatin in an ATP-dependent manner. SWI2/SNF2 is an ATPase whose homologues, BRG1 and hBRM, mediate cell-cycle arrest; the SNF5 homologue, INI1/hSNF5, appears to be a turnout suppressor. A search for INI1-interacting proteins using the two-hybrid system led to the isolation of c-MYC, a transactivator. The c- MYC-INI1 interaction was observed both in vitro and in vivo. The c-MYC basic helix-loop-helix (bHLH) and leucine zipper (Zip) domains and the INI1 repeat 1 (Rpt1) region were required for this interaction, c-MYC-mediated transactivation was inhibited by a deletion fragment of INI1 and the ATPase mutant of BRG1/hSNF2 in a dominant-negative manner contingent upon the presence of the c-MYC bHLH-Zip domain. Our results suggest that the SWI/SNF complex is necessary for c-MYC-mediated transactivation and that the c-MYC- INI1 interaction helps recruit the complex.

Original languageEnglish (US)
Pages (from-to)102-105
Number of pages4
JournalNature Genetics
Volume22
Issue number1
DOIs
StatePublished - May 1999

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Transcriptional Activation
Adenosine Triphosphatases
Helix-Loop-Helix Motifs
Multiprotein Complexes
Leucine Zippers
Chromatin Assembly and Disassembly
Trans-Activators
Gene Expression Regulation
Cell Cycle Checkpoints
Chromatin
Adenosine Triphosphate
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function. / Cheng, S. W Grace; Davies, Kelvin; Yung, Eric; Beltran, Ralph J.; Yu, Jin; Kalpana, Ganjam V.

In: Nature Genetics, Vol. 22, No. 1, 05.1999, p. 102-105.

Research output: Contribution to journalArticle

Cheng, S. W Grace ; Davies, Kelvin ; Yung, Eric ; Beltran, Ralph J. ; Yu, Jin ; Kalpana, Ganjam V. / c-MYC interacts with INI1/hSNF5 and requires the SWI/SNF complex for transactivation function. In: Nature Genetics. 1999 ; Vol. 22, No. 1. pp. 102-105.
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