c-myc-Dependent hepatoma cell apoptosis results from oxidative stress and not a deficiency of growth factors

Yang Xu, Quynh Nguyen, Danica Lo, Mark J. Czaja

Research output: Contribution to journalArticle

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Abstract

Expression of c-myc regulates apoptotic cell death in the human hepatoma cell line HuH-7 during culture in serum-free medium (SFM) plus zinc. To understand the mechanism of this c-myc effect, the ability of various serum-contained factors to prevent apoptosis was determined. Apoptosis was not inhibited by growth factors and was even accelerated by supplementation with insulin-like growth factor I or insulin. Cell death was prevented by SFM supplementation with the amino acid glutamine but not serine or asparagine. Improved cell survival with glutamine was associated with increased levels of glutathione (GSH). In HuH-7 cells cultured in SFM plus zinc, c-myc expression led to decreased levels of GSH, and elevated intracellular levels of hydrogen peroxide (H2O2). Cell death induced by c-myc expression was inhibited by the addition of catalase or dimethyl sulfoxide, a hydroxyl radical scavenger, or by increased intracellular expression of catalase. In contrast to findings in fibroblasts, c-myc-dependent apoptosis during serum deprivation in HuH-7 hepatoma cells was unrelated to a loss of growth factors. Apoptosis resulted from H2O2-mediated oxidative stress with associated glutamine dependent intracellular GSH depletion.

Original languageEnglish (US)
Pages (from-to)192-199
Number of pages8
JournalJournal of Cellular Physiology
Volume170
Issue number2
DOIs
StatePublished - Feb 1997

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Oxidative stress
Hepatocellular Carcinoma
Intercellular Signaling Peptides and Proteins
Serum-Free Culture Media
Oxidative Stress
Cell death
Glutamine
Apoptosis
Cell Death
Catalase
Zinc
Cells
Asparagine
Fibroblasts
Dimethyl Sulfoxide
Serum
Insulin-Like Growth Factor I
Cell culture
Hydroxyl Radical
Serine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

c-myc-Dependent hepatoma cell apoptosis results from oxidative stress and not a deficiency of growth factors. / Xu, Yang; Nguyen, Quynh; Lo, Danica; Czaja, Mark J.

In: Journal of Cellular Physiology, Vol. 170, No. 2, 02.1997, p. 192-199.

Research output: Contribution to journalArticle

Xu, Yang ; Nguyen, Quynh ; Lo, Danica ; Czaja, Mark J. / c-myc-Dependent hepatoma cell apoptosis results from oxidative stress and not a deficiency of growth factors. In: Journal of Cellular Physiology. 1997 ; Vol. 170, No. 2. pp. 192-199.
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