C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice

Aning Sun, Gaiyan Ren, Chao Deng, Jingjing Zhang, Xiaoping Luo, Xiaojun Wu, Sridhar Mani, Wei Dou, Zhengtao Wang

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.

Original languageEnglish (US)
Pages (from-to)418-430
Number of pages13
JournalJournal of Functional Foods
Volume21
DOIs
StatePublished - Mar 1 2016

Fingerprint

NF-kappa B
inflammatory bowel disease
mitogen-activated protein kinase
Inflammatory Bowel Diseases
Flavonoids
sodium sulfate
flavonoids
dextran
mice
Dextran Sulfate
Mitogen-Activated Protein Kinases
myeloperoxidase
colitis
luciferase
Up-Regulation
Interleukin-1 Receptor-Associated Kinases
Myeloid Differentiation Factor 88
herbs
inactivation
disease control

Keywords

  • DSS-induced colitis
  • MAPK
  • NF-kB
  • Orientin
  • TLR4

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice. / Sun, Aning; Ren, Gaiyan; Deng, Chao; Zhang, Jingjing; Luo, Xiaoping; Wu, Xiaojun; Mani, Sridhar; Dou, Wei; Wang, Zhengtao.

In: Journal of Functional Foods, Vol. 21, 01.03.2016, p. 418-430.

Research output: Contribution to journalArticle

Sun, Aning ; Ren, Gaiyan ; Deng, Chao ; Zhang, Jingjing ; Luo, Xiaoping ; Wu, Xiaojun ; Mani, Sridhar ; Dou, Wei ; Wang, Zhengtao. / C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice. In: Journal of Functional Foods. 2016 ; Vol. 21. pp. 418-430.
@article{560a7512c5024c0c9a962b9179ca9abd,
title = "C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice",
abstract = "Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.",
keywords = "DSS-induced colitis, MAPK, NF-kB, Orientin, TLR4",
author = "Aning Sun and Gaiyan Ren and Chao Deng and Jingjing Zhang and Xiaoping Luo and Xiaojun Wu and Sridhar Mani and Wei Dou and Zhengtao Wang",
year = "2016",
month = "3",
day = "1",
doi = "10.1016/j.jff.2015.12.029",
language = "English (US)",
volume = "21",
pages = "418--430",
journal = "Journal of Functional Foods",
issn = "1756-4646",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - C-glycosyl flavonoid orientin improves chemically induced inflammatory bowel disease in mice

AU - Sun, Aning

AU - Ren, Gaiyan

AU - Deng, Chao

AU - Zhang, Jingjing

AU - Luo, Xiaoping

AU - Wu, Xiaojun

AU - Mani, Sridhar

AU - Dou, Wei

AU - Wang, Zhengtao

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.

AB - Orientin is a C-glycosyl flavonoid found abundantly in some fruits and herbs. The present study demonstrated that orientin (50 and 100 mg/kg/d) alleviated the severity of dextran sulphate sodium (DSS)-induced colitis in mice by decreasing the activity of myeloperoxidase (MPO) and the production of cytokines. Concomitant up-regulation of toll-like receptor (TLR)-after DSS exposure was associated with an increase in the activation of myeloid differentiation factor 88 (MyD88), IL-receptor-associated kinase (IRAK)-1, nuclear factor kappa B (NF-kB) p65, as well as the mitogen-activated protein kinase (MAPK) molecules (ERK1/2, JNK and p38), and this upregulation was reversed by orientin administration. Moreover, orientin inhibited the nuclear translocation of NF-kB p65, the activity of NF-kB-luciferase, and the expression of NF-kB target genes. These findings suggest that orientin attenuates experimental inflammatory bowel disease (IBD) via suppression of TLR4 and inactivation of NF-kB and MAPK pathways.

KW - DSS-induced colitis

KW - MAPK

KW - NF-kB

KW - Orientin

KW - TLR4

UR - http://www.scopus.com/inward/record.url?scp=84957895004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957895004&partnerID=8YFLogxK

U2 - 10.1016/j.jff.2015.12.029

DO - 10.1016/j.jff.2015.12.029

M3 - Article

AN - SCOPUS:84957895004

VL - 21

SP - 418

EP - 430

JO - Journal of Functional Foods

JF - Journal of Functional Foods

SN - 1756-4646

ER -