TY - JOUR
T1 - Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling
AU - Diaz-Griffero, Felipe
AU - Hoschander, Steven A.
AU - Brojatsch, Jürgen
PY - 2003/12
Y1 - 2003/12
N2 - Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.
AB - Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.
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U2 - 10.1128/JVI.77.23.12552-12561.2003
DO - 10.1128/JVI.77.23.12552-12561.2003
M3 - Article
C2 - 14610178
AN - SCOPUS:0242492811
SN - 0022-538X
VL - 77
SP - 12552
EP - 12561
JO - Journal of virology
JF - Journal of virology
IS - 23
ER -