Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling

Felipe Diaz-Griffero, Steven A. Hoschander, Jürgen Brojatsch

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.

Original languageEnglish (US)
Pages (from-to)12552-12561
Number of pages10
JournalJournal of Virology
Volume77
Issue number23
DOIs
StatePublished - Dec 2003

Fingerprint

Avian Leukosis Virus
Avian leukosis virus
Infection
infection
cell death
Cell Death
apoptosis
Apoptosis
cells
Superinfection
receptors
tumor necrosis factors
Tumor Necrosis Factor Receptors
caspases
Caspases
B-lymphocytes
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling. / Diaz-Griffero, Felipe; Hoschander, Steven A.; Brojatsch, Jürgen.

In: Journal of Virology, Vol. 77, No. 23, 12.2003, p. 12552-12561.

Research output: Contribution to journalArticle

Diaz-Griffero, Felipe ; Hoschander, Steven A. ; Brojatsch, Jürgen. / Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling. In: Journal of Virology. 2003 ; Vol. 77, No. 23. pp. 12552-12561.
@article{5f1687357b1548af9adaabb11115e007,
title = "Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling",
abstract = "Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.",
author = "Felipe Diaz-Griffero and Hoschander, {Steven A.} and J{\"u}rgen Brojatsch",
year = "2003",
month = "12",
doi = "10.1128/JVI.77.23.12552-12561.2003",
language = "English (US)",
volume = "77",
pages = "12552--12561",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",

}

TY - JOUR

T1 - Bystander Killing during Avian Leukosis Virus Subgroup B Infection Requires TVBS3 Signaling

AU - Diaz-Griffero, Felipe

AU - Hoschander, Steven A.

AU - Brojatsch, Jürgen

PY - 2003/12

Y1 - 2003/12

N2 - Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.

AB - Cell killing by avian leukosis virus subgroup B (ALV-B) in cultures has been extensively studied, but the molecular basis of this process has not been established. Here we show that superinfection, which has been linked to cell killing by ALV-B, plays no crucial role in cell death induction. Instead, we show that signaling by the ALV-B receptor, TVBS3, a member of the tumor necrosis factor receptor family, is essential for ALV-B-mediated cell death. TVBS3 activated caspase-dependent apoptosis during ALV-B infection. Strikingly, apoptosis induction occurred predominantly in uninfected cells, while ALV-B-infected cells were protected against cell death. This bystander killing phenomenon was reproduced in a virus-free system by cocultivating ALV-B Env-expressing cells with TVBS3-expressing cells. Taken together, our results indicated that ALV-B-mediated apoptosis is triggered by ALV-B Env-TVBS3 interactions.

UR - http://www.scopus.com/inward/record.url?scp=0242492811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0242492811&partnerID=8YFLogxK

U2 - 10.1128/JVI.77.23.12552-12561.2003

DO - 10.1128/JVI.77.23.12552-12561.2003

M3 - Article

VL - 77

SP - 12552

EP - 12561

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 23

ER -