Bxz1, a new generalized transducing phage for mycobacteria

Sunhee Lee, Jordan Kriakov, Catherine Vilcheze, Zhiyan Dai, Graham F. Hatfull, William R. Jacobs

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

We have isolated and characterized a new generalized transducing phage, Bxz1, from soil sampling at a neighboring Wildlife Preservation Park. The hosts of the phage, measured by the formation of plaques, include fast growing Mycobacterium smegmatis and Mycobacterium vaccae. Bxz1 is capable of transducing chromosomal markers, point mutations, and plasmids at frequencies ranging from 10 -8 to 10 -6 per plaque forming unit between strains of M. smegmatis. We also demonstrated cotransduction of a transposon insertion linked to a point mutation of the ndh gene.

Original languageEnglish (US)
Pages (from-to)271-276
Number of pages6
JournalFEMS Microbiology Letters
Volume241
Issue number2
DOIs
StatePublished - Dec 15 2004
Externally publishedYes

Keywords

  • Mycobacteria
  • Transducing phage

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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