Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells

Sandra Maier, M. Cecilia Daroqui, Stefan Scherer, Stefan Roepcke, Anna Velcich, Shailesh M. Shenoy, Robert H. Singer, Leonard H. Augenlicht

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In stimulating maturation of colonic carcinoma cells, the short chain fatty acid butyrate, and 1α,25-dihydroxyvitamin D3, were shown to attenuate transcription of the cyclin D1 gene, giving rise to truncated transcripts of this locus. Moreover, a sequence which is highly conserved in the human, mouse, rat, and dog genome was found in the 4 kb long intron 3 of the human cyclin D1 gene, and is capable of forming a hairpin structure similar to that of microRNA precursors. The expression of this sequence is also decreased by the attenuation. Thus, the transcriptional attenuation at the cyclin D1 locus not only down-regulates the expression of this key gene in mucosal cell maturation and tumorigenesis, but may also abrogate the generation of a molecule that encompasses this conserved sequence in cyclin D1 intron 3.

Original languageEnglish (US)
Pages (from-to)638-642
Number of pages5
JournalJournal of Cellular Physiology
Volume218
Issue number3
DOIs
StatePublished - Mar 2009

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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