Bucky Ball Organizes Germ Plasm Assembly in Zebrafish

Franck Bontems, Amandine Stein, Florence Marlow, Jacqueline Lyautey, Tripti Gupta, Mary C. Mullins, Roland Dosch

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

In many animals, gamete formation during embryogenesis is specified by maternal cytoplasmic determinants termed germ plasm [1, 2]. During oogenesis, germ plasm forms a distinct cellular structure such as pole plasm in Drosophila or the Balbiani body, an aggregate of organelles also found in mammals [3-10]. However, in vertebrates, the key regulators of germ plasm assembly are largely unknown. Here, we show that, at the beginning of zebrafish oogenesis, the germ plasm defect in bucky ball (buc) mutants precedes the loss of polarity, indicating that Buc primarily controls Balbiani body formation. Moreover, we molecularly identify the buc gene, which is exclusively expressed in the ovary with a novel, dynamic mRNA localization pattern first detectable within the Balbiani body. We find that a Buc-GFP fusion localizes to the Balbiani body during oogenesis and with the germ plasm during early embryogenesis, consistent with a role in germ plasm formation. Interestingly, overexpression of buc seems to generate ectopic germ cells in the zebrafish embryo. Because we discovered buc homologs in many vertebrate genomes, including mammals, these results identify buc as the first gene necessary and sufficient for germ plasm organization in vertebrates.

Original languageEnglish (US)
Pages (from-to)414-422
Number of pages9
JournalCurrent Biology
Volume19
Issue number5
DOIs
StatePublished - Mar 10 2009
Externally publishedYes

Fingerprint

fullerene
Oogenesis
Zebrafish
Danio rerio
Vertebrates
germplasm
Mammals
Genes
Germ Cells
Embryonic Development
oogenesis
Cellular Structures
Organelles
vertebrates
Drosophila
Ovary
Poles
Animals
Fusion reactions
Embryonic Structures

Keywords

  • DEVBIO
  • RNA

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bontems, F., Stein, A., Marlow, F., Lyautey, J., Gupta, T., Mullins, M. C., & Dosch, R. (2009). Bucky Ball Organizes Germ Plasm Assembly in Zebrafish. Current Biology, 19(5), 414-422. https://doi.org/10.1016/j.cub.2009.01.038

Bucky Ball Organizes Germ Plasm Assembly in Zebrafish. / Bontems, Franck; Stein, Amandine; Marlow, Florence; Lyautey, Jacqueline; Gupta, Tripti; Mullins, Mary C.; Dosch, Roland.

In: Current Biology, Vol. 19, No. 5, 10.03.2009, p. 414-422.

Research output: Contribution to journalArticle

Bontems, F, Stein, A, Marlow, F, Lyautey, J, Gupta, T, Mullins, MC & Dosch, R 2009, 'Bucky Ball Organizes Germ Plasm Assembly in Zebrafish', Current Biology, vol. 19, no. 5, pp. 414-422. https://doi.org/10.1016/j.cub.2009.01.038
Bontems F, Stein A, Marlow F, Lyautey J, Gupta T, Mullins MC et al. Bucky Ball Organizes Germ Plasm Assembly in Zebrafish. Current Biology. 2009 Mar 10;19(5):414-422. https://doi.org/10.1016/j.cub.2009.01.038
Bontems, Franck ; Stein, Amandine ; Marlow, Florence ; Lyautey, Jacqueline ; Gupta, Tripti ; Mullins, Mary C. ; Dosch, Roland. / Bucky Ball Organizes Germ Plasm Assembly in Zebrafish. In: Current Biology. 2009 ; Vol. 19, No. 5. pp. 414-422.
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