BSAP (Pax5)-importin α1 (Rch1) interaction identifies a nuclear localization sequence

Cecilia R. Kovac, Alexander Emelyanov, Mallika Singh, Nasrin Ashouian, Barbara K. Birshtein

Research output: Contribution to journalArticle

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Abstract

BSAP (Pax5) is an essential transcription factor for early B cell and central nervous system development. In later B cell development, BSAP has been implicated in the regulation of 3' Ig enhancers and a number of B cell- specific genes. Previous studies have suggested a role for BSAP-interacting proteins in the regulation of the function of BSAP. Using the yeast two- hybrid system, we identified importin α1 (Rch1) as a BSAP-interacting protein. Importin α proteins have been shown to escort proteins into the nucleus through interaction with a nuclear localization signal (NLS), composed of short stretches of basic amino acids. A predicted NLS in BSAP (NKRKRDE, located at amino acids 195-201 in the central domain) was confirmed to be essential for interaction with importin α1 by the yeast two-hybrid assay. Physical interaction between BSAP and importin α1 was detected in vitro by a glutathione S-transferase (GST) pulldown assay. The NLS sequence in BSAP conferred nuclear localization to green fluorescent protein (GFP)- BSAP fusion proteins. Although the N-terminal paired (DNA-binding) domain of BSAP also conferred nuclear localization when coupled to green fluorescent protein, this domain did not bind to importin α1 in the yeast two-hybrid assay. The NLS sequence in the central domain of BSAP binds to the C-terminal 98-amino acid fragment of importin α1.

Original languageEnglish (US)
Pages (from-to)16752-16757
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number22
DOIs
StatePublished - Jun 2 2000

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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