TY - JOUR
T1 - Broth microdilution in vitro screening
T2 - An easy and fast method to detect new antifungal compounds
AU - De-Souza-Silva, Calliandra Maria
AU - Guilhelmelli, Fernanda
AU - Zamith-Miranda, Daniel
AU - De Oliveira, Marco Antônio
AU - Nosanchuk, Joshua Daniel
AU - Silva-Pereira, Ildinete
AU - Albuquerque, Patrícia
N1 - Publisher Copyright:
© 2018 Journal of Visualized Experiments.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
AB - Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
KW - Antifungal screening
KW - Antimicrobial peptide
KW - Broth microdilution method
KW - CLSI M27-A3
KW - Candida spp
KW - Cryptococcus spp
UR - http://www.scopus.com/inward/record.url?scp=85042443773&partnerID=8YFLogxK
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U2 - 10.3791/57127
DO - 10.3791/57127
M3 - Article
C2 - 29553507
AN - SCOPUS:85042443773
SN - 1940-087X
VL - 2018
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 132
M1 - e57127
ER -