Broth microdilution in vitro screening: An easy and fast method to detect new antifungal compounds

Calliandra Maria De-Souza-Silva, Fernanda Guilhelmelli, Daniel Zamith-Miranda, Marco Antônio De Oliveira, Joshua Daniel Nosanchuk, Ildinete Silva-Pereira, Patrícia Albuquerque

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.

Original languageEnglish (US)
Article numbere57127
JournalJournal of Visualized Experiments
Issue number132
StatePublished - Feb 7 2018


  • Antifungal screening
  • Antimicrobial peptide
  • Broth microdilution method
  • CLSI M27-A3
  • Candida spp
  • Cryptococcus spp

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)


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