Abstract
Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
| Original language | English (US) |
|---|---|
| Article number | e57127 |
| Journal | Journal of Visualized Experiments |
| Volume | 2018 |
| Issue number | 132 |
| DOIs | |
| State | Published - Feb 7 2018 |
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Keywords
- Antifungal screening
- Antimicrobial peptide
- Broth microdilution method
- Candida spp
- CLSI M27-A3
- Cryptococcus spp
ASJC Scopus subject areas
- Neuroscience(all)
- Chemical Engineering(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
Cite this
Broth microdilution in vitro screening : An easy and fast method to detect new antifungal compounds. / De-Souza-Silva, Calliandra Maria; Guilhelmelli, Fernanda; Zamith-Miranda, Daniel; De Oliveira, Marco Antônio; Nosanchuk, Joshua D.; Silva-Pereira, Ildinete; Albuquerque, Patrícia.
In: Journal of Visualized Experiments, Vol. 2018, No. 132, e57127, 07.02.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Broth microdilution in vitro screening
T2 - An easy and fast method to detect new antifungal compounds
AU - De-Souza-Silva, Calliandra Maria
AU - Guilhelmelli, Fernanda
AU - Zamith-Miranda, Daniel
AU - De Oliveira, Marco Antônio
AU - Nosanchuk, Joshua D.
AU - Silva-Pereira, Ildinete
AU - Albuquerque, Patrícia
PY - 2018/2/7
Y1 - 2018/2/7
N2 - Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
AB - Fungal infections have become an important medical condition in the last decades, but the number of available antifungal drugs is limited. In this scenario, the search for new antifungal drugs is necessary. The protocol reported here details a method to screen peptides for their antifungal properties. It is based on the broth microdilution susceptibility test from the Clinical and Laboratory Standards Institute (CLSI) M27-A3 guidelines with modifications to suit the research of antimicrobial peptides as potential new antifungals. This protocol describes a functional assay to evaluate the activity of antifungal compounds and may be easily modified to suit any particular class of molecules under investigation. Since the assays are performed in 96-well plates using small volumes, a large-scale screening can be completed in a short amount of time, especially if carried out in an automation setting. This procedure illustrates how a standardized and adjustable clinical protocol can help the bench-work pursuit of new molecules to improve the therapy of fungal diseases.
KW - Antifungal screening
KW - Antimicrobial peptide
KW - Broth microdilution method
KW - Candida spp
KW - CLSI M27-A3
KW - Cryptococcus spp
UR - http://www.scopus.com/inward/record.url?scp=85042443773&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85042443773&partnerID=8YFLogxK
U2 - 10.3791/57127
DO - 10.3791/57127
M3 - Article
C2 - 29553507
AN - SCOPUS:85042443773
VL - 2018
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 132
M1 - e57127
ER -