Brightness-equalized quantum dots

Sung Jun Lim, Mohammad U. Zahid, Phuong Le, Liang Ma, David R. Entenberg, Allison S. Harney, John S. Condeelis, Andrew M. Smith

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

As molecular labels for cells and tissues, fluorescent probes have shaped our understanding of biological structures and processes. However, their capacity for quantitative analysis is limited because photon emission rates from multicolour fluorophores are dissimilar, unstable and often unpredictable, which obscures correlations between measured fluorescence and molecular concentration. Here we introduce a new class of light-emitting quantum dots with tunable and equalized fluorescence brightness across a broad range of colours. The key feature is independent tunability of emission wavelength, extinction coefficient and quantum yield through distinct structural domains in the nanocrystal. Precise tuning eliminates a 100-fold red-to-green brightness mismatch of size-tuned quantum dots at the ensemble and single-particle levels, which substantially improves quantitative imaging accuracy in biological tissue. We anticipate that these materials engineering principles will vastly expand the optical engineering landscape of fluorescent probes, facilitate quantitative multicolour imaging in living tissue and improve colour tuning in light-emitting devices.

Original languageEnglish (US)
Article number8210
JournalNature Communications
Volume6
DOIs
StatePublished - Oct 5 2015

Fingerprint

Quantum Dots
Semiconductor quantum dots
Luminance
brightness
quantum dots
Tissue
Fluorescent Dyes
Color
Tuning
Fluorescence
tuning
engineering
Biological Phenomena
Optical engineering
color
Imaging techniques
Light
Light extinction
fluorescence
Fluorophores

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Lim, S. J., Zahid, M. U., Le, P., Ma, L., Entenberg, D. R., Harney, A. S., ... Smith, A. M. (2015). Brightness-equalized quantum dots. Nature Communications, 6, [8210]. https://doi.org/10.1038/ncomms9210

Brightness-equalized quantum dots. / Lim, Sung Jun; Zahid, Mohammad U.; Le, Phuong; Ma, Liang; Entenberg, David R.; Harney, Allison S.; Condeelis, John S.; Smith, Andrew M.

In: Nature Communications, Vol. 6, 8210, 05.10.2015.

Research output: Contribution to journalArticle

Lim, SJ, Zahid, MU, Le, P, Ma, L, Entenberg, DR, Harney, AS, Condeelis, JS & Smith, AM 2015, 'Brightness-equalized quantum dots', Nature Communications, vol. 6, 8210. https://doi.org/10.1038/ncomms9210
Lim SJ, Zahid MU, Le P, Ma L, Entenberg DR, Harney AS et al. Brightness-equalized quantum dots. Nature Communications. 2015 Oct 5;6. 8210. https://doi.org/10.1038/ncomms9210
Lim, Sung Jun ; Zahid, Mohammad U. ; Le, Phuong ; Ma, Liang ; Entenberg, David R. ; Harney, Allison S. ; Condeelis, John S. ; Smith, Andrew M. / Brightness-equalized quantum dots. In: Nature Communications. 2015 ; Vol. 6.
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