Bright and stable near-infrared fluorescent protein for in vivo imaging

Grigory S. Filonov, Kiryl D. Piatkevich, Li Min Ting, Jinghang Zhang, Kami Kim, Vladislav V. Verkhusha

Research output: Contribution to journalArticlepeer-review

449 Scopus citations

Abstract

Imaging biological processes in mammalian tissues will be facilitated by fluorescent probes with excitation and emission bands within the near-infrared optical window of high transparency. Here we report a phytochrome-based near-infrared fluorescent protein (iRFP) with excitation and emission maxima at 690 nm and 713 nm, respectively. iRFP does not require an exogenous supply of the chromophore biliverdin and has higher effective brightness, intracellular stability and photostability than earlier phytochrome-derived fluorescent probes. Compared with far-red GFP-like proteins, iRFP has a substantially higher signal-to-background ratio in a mouse model due to its infrared-shifted spectra.

Original languageEnglish (US)
Pages (from-to)757-761
Number of pages5
JournalNature biotechnology
Volume29
Issue number8
DOIs
StatePublished - Aug 1 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

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