Breast cancer cells isolated by chemotaxis from primary tumors show increased survival and resistance to chemotherapy

Sumanta Goswami, Weigang Wang, Jeffrey B. Wyckoff, John S. Condeelis

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

In this study, we have collected a migratory population of carcinoma cells by chemotaxis to epidermal growth factor-containing microneedles held in the primary tumor. The collected cells were subjected to microarray analysis for differential gene expression. The results show that anti-apoptotic genes are up-regulated and pro-apoptotic genes are down-regulated coordinately in the migratory subpopulation. Induction of apoptosis by doxorubicin, cisplatin, and etoposide in these cells demonstrates that they exhibit a lower drug-induced apoptotic index and lower cell death compared with carcinoma cells of the whole tumor. Our study indicates, for the first time, the capability of using a rat alograft model for evaluating the apoptotic status of a migratory subpopulation of tumor cells and the ability to study their resistance to chemotherapeutic agents directly. In audition, these results indicate that tumor cells that are chemotactic and migratory in response to epidermal growth factor in the primary tumor have a survival advantage over stationary tumor cells.

Original languageEnglish (US)
Pages (from-to)7664-7667
Number of pages4
JournalCancer Research
Volume64
Issue number21
DOIs
StatePublished - Nov 1 2004

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Chemotaxis
Breast Neoplasms
Drug Therapy
Neoplasms
Epidermal Growth Factor
Carcinoma
Aptitude
Etoposide
Microarray Analysis
Doxorubicin
Cisplatin
Hearing
Genes
Cell Death
Apoptosis
Gene Expression
Pharmaceutical Preparations
Population

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Breast cancer cells isolated by chemotaxis from primary tumors show increased survival and resistance to chemotherapy. / Goswami, Sumanta; Wang, Weigang; Wyckoff, Jeffrey B.; Condeelis, John S.

In: Cancer Research, Vol. 64, No. 21, 01.11.2004, p. 7664-7667.

Research output: Contribution to journalArticle

Goswami, Sumanta ; Wang, Weigang ; Wyckoff, Jeffrey B. ; Condeelis, John S. / Breast cancer cells isolated by chemotaxis from primary tumors show increased survival and resistance to chemotherapy. In: Cancer Research. 2004 ; Vol. 64, No. 21. pp. 7664-7667.
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