BRCA1 supports XIST RNA concentration on the inactive X chromosome

Shridar Ganesan, Daniel P. Silver, Roger A. Greenberg, Dror Avni, Ronny Drapkin, Alexander Miron, Samuel C. Mok, Voahangy Randrianarison, Steven Brodie, Jennifer Salstrom, Theodore P. Rasmussen, Ann Klimke, Christine Marrese, York Marahrens, Chu Xia Deng, Jean Feunteun, David M. Livingston

Research output: Contribution to journalArticle

237 Citations (Scopus)

Abstract

BRCA1, a breast and ovarian tumor suppressor, colocalizes with markers of the inactive X chromosome (Xi) on Xi in female somatic cells and associates with XIST RNA, as detected by chromatin immunoprecipitation. Breast and ovarian carcinoma cells lacking BRCA1 show evidence of defects in Xi chromatin structure. Reconstitution of BRCA1-deficient cells with wt BRCA1 led to the appearance of focal XIST RNA staining without altering XIST abundance. Inhibiting BRCA1 synthesis in a suitable reporter line led to increased expression of an otherwise silenced Xi-located GFP transgene. These observations suggest that loss of BRCA1 in female cells may lead to Xi perturbation and destabilization of its silenced state.

Original languageEnglish (US)
Pages (from-to)393-405
Number of pages13
JournalCell
Volume111
Issue number3
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

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X Chromosome
Chromosomes
Chromatin
RNA
Tumors
Cells
Breast Neoplasms
Defects
Chromatin Immunoprecipitation
Transgenes
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Ganesan, S., Silver, D. P., Greenberg, R. A., Avni, D., Drapkin, R., Miron, A., ... Livingston, D. M. (2002). BRCA1 supports XIST RNA concentration on the inactive X chromosome. Cell, 111(3), 393-405. https://doi.org/10.1016/S0092-8674(02)01052-8

BRCA1 supports XIST RNA concentration on the inactive X chromosome. / Ganesan, Shridar; Silver, Daniel P.; Greenberg, Roger A.; Avni, Dror; Drapkin, Ronny; Miron, Alexander; Mok, Samuel C.; Randrianarison, Voahangy; Brodie, Steven; Salstrom, Jennifer; Rasmussen, Theodore P.; Klimke, Ann; Marrese, Christine; Marahrens, York; Deng, Chu Xia; Feunteun, Jean; Livingston, David M.

In: Cell, Vol. 111, No. 3, 01.11.2002, p. 393-405.

Research output: Contribution to journalArticle

Ganesan, S, Silver, DP, Greenberg, RA, Avni, D, Drapkin, R, Miron, A, Mok, SC, Randrianarison, V, Brodie, S, Salstrom, J, Rasmussen, TP, Klimke, A, Marrese, C, Marahrens, Y, Deng, CX, Feunteun, J & Livingston, DM 2002, 'BRCA1 supports XIST RNA concentration on the inactive X chromosome', Cell, vol. 111, no. 3, pp. 393-405. https://doi.org/10.1016/S0092-8674(02)01052-8
Ganesan S, Silver DP, Greenberg RA, Avni D, Drapkin R, Miron A et al. BRCA1 supports XIST RNA concentration on the inactive X chromosome. Cell. 2002 Nov 1;111(3):393-405. https://doi.org/10.1016/S0092-8674(02)01052-8
Ganesan, Shridar ; Silver, Daniel P. ; Greenberg, Roger A. ; Avni, Dror ; Drapkin, Ronny ; Miron, Alexander ; Mok, Samuel C. ; Randrianarison, Voahangy ; Brodie, Steven ; Salstrom, Jennifer ; Rasmussen, Theodore P. ; Klimke, Ann ; Marrese, Christine ; Marahrens, York ; Deng, Chu Xia ; Feunteun, Jean ; Livingston, David M. / BRCA1 supports XIST RNA concentration on the inactive X chromosome. In: Cell. 2002 ; Vol. 111, No. 3. pp. 393-405.
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