Branched amphiphilic peptide capsules: Cellular uptake and retention of encapsulated solutes

Pinakin Sukthankar, L. Adriana Avila, Susan K. Whitaker, Takeo Iwamoto, Alfred Morgenstern, Christos Apostolidis, Ke Liu, Robert P. Hanzlik, Ekaterina Dadachova, John M. Tomich

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Branched amphiphilic peptide capsules (BAPCs) are peptide nano-spheres comprised of equimolar proportions of two branched peptide sequences bis(FLIVI)-K-KKKK and bis(FLIVIGSII)-K-KKKK that self-assemble to form bilayer delimited capsules. In two recent publications we described the lipid analogous characteristics of our BAPCs, examined their initial assembly, mode of fusion, solute encapsulation, and resizing and delineated their capability to be maintained at a specific size by storing them at 4 °C. In this report we describe the stability, size limitations of encapsulation, cellular localization, retention and, bio-distribution of the BAPCs in vivo. The ability of our constructs to retain alpha particle emitting radionuclides without any apparent leakage and their persistence in the peri-nuclear region of the cell for extended periods of time, coupled with their ease of preparation and potential tune-ability, makes them attractive as biocompatible carriers for targeted cancer therapy using particle emitting radioisotopes. This article is part of a Special Issue entitled: Interfacially Active Peptides and Proteins. Guest Editors: William C. Wimley and Kalina Hristova.

Original languageEnglish (US)
Pages (from-to)2296-2305
Number of pages10
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1838
Issue number9
DOIs
StatePublished - Sep 2014

Keywords

  • Alpha particle therapy
  • BAPC
  • Nano-capsule
  • Peptide capsule
  • Self-assembling peptide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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    Sukthankar, P., Avila, L. A., Whitaker, S. K., Iwamoto, T., Morgenstern, A., Apostolidis, C., Liu, K., Hanzlik, R. P., Dadachova, E., & Tomich, J. M. (2014). Branched amphiphilic peptide capsules: Cellular uptake and retention of encapsulated solutes. Biochimica et Biophysica Acta - Biomembranes, 1838(9), 2296-2305. https://doi.org/10.1016/j.bbamem.2014.02.005