BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints

Shailesh Agarwal, Shawn J. Loder, Cameron Brownley, Oluwatobi Eboda, Jonathan R. Peterson, Satoru Hayano, Bingruo Wu, Bin Zhao, Vesa Kaartinen, Victor C. Wong, Yuji Mishina, Benjamin Levi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

BMP signaling mediated by ACVR1 plays a critical role for development of multiple structures including the cardiovascular and skeletal systems. While deficient ACVR1 signaling impairs normal embryonic development, hyperactive ACVR1 function (R206H in humans and Q207D mutation in mice, ca-ACVR1) results in formation of heterotopic ossification (HO). We developed a mouse line, which conditionally expresses ca-ACVR1 with Nfatc1-Cre<sup>+</sup> transgene. Mutant mice developed ectopic cartilage and bone at the distal joints of the extremities including the interphalangeal joints and hind limb ankles as early as P4 in the absence of trauma or exogenous bone morphogenetic protein (BMP) administration. Micro-CT showed that even at later time points (up to P40), cartilage and bone development persisted at the affected joints most prominently in the ankle. Interestingly, this phenotype was not present in areas of bone outside of the joints - tibia are normal in mutants and littermate controls away from the ankle. These findings demonstrate that this model may allow for further studies of heterotopic ossification, which does not require the use of stem cells, direct trauma or activation with exogenous Cre gene administration.

Original languageEnglish (US)
Pages (from-to)202-209
Number of pages8
JournalDevelopmental Biology
Volume400
Issue number2
DOIs
StatePublished - 2015

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Type I Activin Receptors
Bone Morphogenetic Proteins
Osteogenesis
Extremities
Joints
Ankle
Heterotopic Ossification
Cartilage
Bone and Bones
Bone Development
Wounds and Injuries
Cardiovascular System
Tibia
Transgenes
Embryonic Development
Stem Cells
Phenotype
Mutation
Genes

Keywords

  • Acvr1
  • ALK2
  • BMP receptor
  • Bone
  • Cartilage
  • Endochondral ossification
  • Fibrodysplasia ossificans progressive
  • FOP
  • Heterotopic ossification
  • Nfatc1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints. / Agarwal, Shailesh; Loder, Shawn J.; Brownley, Cameron; Eboda, Oluwatobi; Peterson, Jonathan R.; Hayano, Satoru; Wu, Bingruo; Zhao, Bin; Kaartinen, Vesa; Wong, Victor C.; Mishina, Yuji; Levi, Benjamin.

In: Developmental Biology, Vol. 400, No. 2, 2015, p. 202-209.

Research output: Contribution to journalArticle

Agarwal, S, Loder, SJ, Brownley, C, Eboda, O, Peterson, JR, Hayano, S, Wu, B, Zhao, B, Kaartinen, V, Wong, VC, Mishina, Y & Levi, B 2015, 'BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints', Developmental Biology, vol. 400, no. 2, pp. 202-209. https://doi.org/10.1016/j.ydbio.2015.02.011
Agarwal, Shailesh ; Loder, Shawn J. ; Brownley, Cameron ; Eboda, Oluwatobi ; Peterson, Jonathan R. ; Hayano, Satoru ; Wu, Bingruo ; Zhao, Bin ; Kaartinen, Vesa ; Wong, Victor C. ; Mishina, Yuji ; Levi, Benjamin. / BMP signaling mediated by constitutively active Activin type 1 receptor (ACVR1) results in ectopic bone formation localized to distal extremity joints. In: Developmental Biology. 2015 ; Vol. 400, No. 2. pp. 202-209.
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