Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB)

Donglin Bai, Cristiane Del Corsso, Miduturu Srinivas, David C. Spray

Research output: Contribution to journalArticle

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Abstract

2-Aminoethoxydiphenyl borate (2-APB), an inositol 1,4,5-triphosphate receptor modulator, inhibits capacitive current transients measured in normal rat kidney and human embryonic kidney 293 cells, an indication of blocking gap junction channels between these cells. Here, we used the dual whole-cell patch-clamp method to study the actions of 2-APB on gap junction channels formed by selected connexins expressed in a communication-deficient neuroblastoma cell line (N2A). 2-APB dose-dependently and reversibly blocked junctional currents of connexin (Cx) 50 gap junction channels. The concentration-inhibition curve of 2-APB on the junctional current indicated an IC50 of 3.7 μM, lower than that of most gap junction inhibitors. At a concentration of 20 μM, 2-APB also significantly blocked junctional conductance in cell pairs coupled by Cx26, Cx30, Cx36, Cx40, and Cx45 but did not appreciably affect coupling in cell pairs expressing Cx32, Cx43, and Cx46. Although concentration inhibition curves of 2-APB on Cx36 channels were similar to Cx50 (Cx36; IC50, 3.0 μM), IC50 values were higher for Cx43 (51.6 μM), Cx45 (18.1 μM), and Cx46 (29.4 μM). The blocking action of 2-APB did not substantially alter transjunctional voltage-dependent gating of Cx50 gap junction channels, and recordings from poorly coupled pairs of Cx50-transfected N2A cells indicated that 2-APB reduced gap junction channel open probability without changing the main state single-channel conductance. The differential efficacy of block by 2-APB of gap junction channels formed by different connexins may provide a useful tool that could be exploited in gap junction research to selectively block certain gap junction channel subtypes.

Original languageEnglish (US)
Pages (from-to)1452-1458
Number of pages7
JournalJournal of Pharmacology and Experimental Therapeutics
Volume319
Issue number3
DOIs
StatePublished - 2006

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Gap Junctions
Inhibitory Concentration 50
Connexin 43
Connexins
Kidney
Inositol 1,4,5-Trisphosphate Receptors
2-aminoethoxydiphenyl borate
Neuroblastoma
Communication
Cell Line

ASJC Scopus subject areas

  • Pharmacology

Cite this

Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB). / Bai, Donglin; Del Corsso, Cristiane; Srinivas, Miduturu; Spray, David C.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 319, No. 3, 2006, p. 1452-1458.

Research output: Contribution to journalArticle

Bai, Donglin ; Del Corsso, Cristiane ; Srinivas, Miduturu ; Spray, David C. / Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB). In: Journal of Pharmacology and Experimental Therapeutics. 2006 ; Vol. 319, No. 3. pp. 1452-1458.
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