Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease

B. F. Mackler, L. C. Altman, S. Wahl, David L. Rosenstreich, J. J. Oppenheim, S. E. Mergenhagen

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Thymus derived (T) and bone marrow derived (B) lymphocytes were isolated from human peripheral blood and cultured with various mitogens and antigens. Purified protein derivative of tuberculin stimulated both purified T and B cells from patients with positive skin reactivity to purified protein derivative but did not stimulate nonimmune lymphocytes. Similarly, both T and B lymphocytes from patients with periodontal disease were stimulated to proliferate when incubated with dental plaque, whereas cells from normal individuals without gingivitis were unresponsive. In contrast, one component of plaque, bacterial endotoxins (lipopolysaccharide), minimally stimulated B lymphocytes from both normal or gingivitis patients. T lymphocytes from patients with periodontal disease were also stimulated by plaque antigen to produce chemotactic lymphokine activity (CTX) for human monocytes. B cells purified by the EAC rosetting method nonspecifically produced CTX without concomitant blastogenesis; however, after dissociation of adherent EAC these immune B cells did not spontaneously produce CTX. Lymphokine synthesis by B cells was not dependent on concomitant blastogenesis. Dissociated B cells from periodontitis patients also produced CTX activity after stimulation with dental plaque antigen. Therefore, both T and B lymphocytes, after stimulation with nonendotoxin antigenic components of plaque, proliferated and produced lymphokines, which are presumed to contribute to the pathogenesis of periodontal disease.

Original languageEnglish (US)
Pages (from-to)844-850
Number of pages7
JournalInfection and Immunity
Volume10
Issue number4
StatePublished - 1974
Externally publishedYes

Fingerprint

Lymphokines
Periodontal Diseases
Lymphocyte Activation
B-Lymphocytes
T-Lymphocytes
Dental Plaque
Gingivitis
Antigens
Periodontitis
Tuberculin
Mitogens
Human Activities
Endotoxins
Thymus Gland
Lipopolysaccharides
Monocytes
Bone Marrow
Lymphocytes
Skin

ASJC Scopus subject areas

  • Immunology

Cite this

Mackler, B. F., Altman, L. C., Wahl, S., Rosenstreich, D. L., Oppenheim, J. J., & Mergenhagen, S. E. (1974). Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease. Infection and Immunity, 10(4), 844-850.

Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease. / Mackler, B. F.; Altman, L. C.; Wahl, S.; Rosenstreich, David L.; Oppenheim, J. J.; Mergenhagen, S. E.

In: Infection and Immunity, Vol. 10, No. 4, 1974, p. 844-850.

Research output: Contribution to journalArticle

Mackler, BF, Altman, LC, Wahl, S, Rosenstreich, DL, Oppenheim, JJ & Mergenhagen, SE 1974, 'Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease', Infection and Immunity, vol. 10, no. 4, pp. 844-850.
Mackler, B. F. ; Altman, L. C. ; Wahl, S. ; Rosenstreich, David L. ; Oppenheim, J. J. ; Mergenhagen, S. E. / Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease. In: Infection and Immunity. 1974 ; Vol. 10, No. 4. pp. 844-850.
@article{b65b5c2a23a44a06a52e0006ab453455,
title = "Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease",
abstract = "Thymus derived (T) and bone marrow derived (B) lymphocytes were isolated from human peripheral blood and cultured with various mitogens and antigens. Purified protein derivative of tuberculin stimulated both purified T and B cells from patients with positive skin reactivity to purified protein derivative but did not stimulate nonimmune lymphocytes. Similarly, both T and B lymphocytes from patients with periodontal disease were stimulated to proliferate when incubated with dental plaque, whereas cells from normal individuals without gingivitis were unresponsive. In contrast, one component of plaque, bacterial endotoxins (lipopolysaccharide), minimally stimulated B lymphocytes from both normal or gingivitis patients. T lymphocytes from patients with periodontal disease were also stimulated by plaque antigen to produce chemotactic lymphokine activity (CTX) for human monocytes. B cells purified by the EAC rosetting method nonspecifically produced CTX without concomitant blastogenesis; however, after dissociation of adherent EAC these immune B cells did not spontaneously produce CTX. Lymphokine synthesis by B cells was not dependent on concomitant blastogenesis. Dissociated B cells from periodontitis patients also produced CTX activity after stimulation with dental plaque antigen. Therefore, both T and B lymphocytes, after stimulation with nonendotoxin antigenic components of plaque, proliferated and produced lymphokines, which are presumed to contribute to the pathogenesis of periodontal disease.",
author = "Mackler, {B. F.} and Altman, {L. C.} and S. Wahl and Rosenstreich, {David L.} and Oppenheim, {J. J.} and Mergenhagen, {S. E.}",
year = "1974",
language = "English (US)",
volume = "10",
pages = "844--850",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Blastogenesis and lymphokine synthesis by T and B lymphocytes from patients with periodontal disease

AU - Mackler, B. F.

AU - Altman, L. C.

AU - Wahl, S.

AU - Rosenstreich, David L.

AU - Oppenheim, J. J.

AU - Mergenhagen, S. E.

PY - 1974

Y1 - 1974

N2 - Thymus derived (T) and bone marrow derived (B) lymphocytes were isolated from human peripheral blood and cultured with various mitogens and antigens. Purified protein derivative of tuberculin stimulated both purified T and B cells from patients with positive skin reactivity to purified protein derivative but did not stimulate nonimmune lymphocytes. Similarly, both T and B lymphocytes from patients with periodontal disease were stimulated to proliferate when incubated with dental plaque, whereas cells from normal individuals without gingivitis were unresponsive. In contrast, one component of plaque, bacterial endotoxins (lipopolysaccharide), minimally stimulated B lymphocytes from both normal or gingivitis patients. T lymphocytes from patients with periodontal disease were also stimulated by plaque antigen to produce chemotactic lymphokine activity (CTX) for human monocytes. B cells purified by the EAC rosetting method nonspecifically produced CTX without concomitant blastogenesis; however, after dissociation of adherent EAC these immune B cells did not spontaneously produce CTX. Lymphokine synthesis by B cells was not dependent on concomitant blastogenesis. Dissociated B cells from periodontitis patients also produced CTX activity after stimulation with dental plaque antigen. Therefore, both T and B lymphocytes, after stimulation with nonendotoxin antigenic components of plaque, proliferated and produced lymphokines, which are presumed to contribute to the pathogenesis of periodontal disease.

AB - Thymus derived (T) and bone marrow derived (B) lymphocytes were isolated from human peripheral blood and cultured with various mitogens and antigens. Purified protein derivative of tuberculin stimulated both purified T and B cells from patients with positive skin reactivity to purified protein derivative but did not stimulate nonimmune lymphocytes. Similarly, both T and B lymphocytes from patients with periodontal disease were stimulated to proliferate when incubated with dental plaque, whereas cells from normal individuals without gingivitis were unresponsive. In contrast, one component of plaque, bacterial endotoxins (lipopolysaccharide), minimally stimulated B lymphocytes from both normal or gingivitis patients. T lymphocytes from patients with periodontal disease were also stimulated by plaque antigen to produce chemotactic lymphokine activity (CTX) for human monocytes. B cells purified by the EAC rosetting method nonspecifically produced CTX without concomitant blastogenesis; however, after dissociation of adherent EAC these immune B cells did not spontaneously produce CTX. Lymphokine synthesis by B cells was not dependent on concomitant blastogenesis. Dissociated B cells from periodontitis patients also produced CTX activity after stimulation with dental plaque antigen. Therefore, both T and B lymphocytes, after stimulation with nonendotoxin antigenic components of plaque, proliferated and produced lymphokines, which are presumed to contribute to the pathogenesis of periodontal disease.

UR - http://www.scopus.com/inward/record.url?scp=0016210345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016210345&partnerID=8YFLogxK

M3 - Article

C2 - 4547744

AN - SCOPUS:0016210345

VL - 10

SP - 844

EP - 850

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 4

ER -